Proteins identified in P62 pull-down experiments in control and preconditioned tissue
| Treatment | Name | GI number | Peptides | Unique | Function |
|---|---|---|---|---|---|
| Control | β-Tubulin 2 | 33465435 | 2 | 2 | SCM |
| Control | Na,K-ATPase α subunit 3 | 14349292 | 3 | 2 | Met |
| Control | β-Spectrin | 33303722 | 3 | 3 | SCM |
| Control | Similar to KIAA1185 protein | 34872773 | 2 | 2 | TT (?) |
| Control | CYFIP (cytoplasmic Fragile-X interacting family) (rat homolog) | 34870680 | 2 | 2 | SCM |
| Control | Wave 1 (rat homolog) | 34853415 | 2 | 2 | SCM |
| Control | Sam 68 (src associated in mitosis) | 18426824 | 2 | 2 | TT |
| Preconditioning | Calcineurin (protein phosphatase 2Bα subunit) | 16758910 | 2 | 2 | ST |
| Preconditioning | Creatine kinase | 31542401 | 2 | 2 | Met |
| Preconditioning | Elongation factor-1α | 6856354 | 4 | 2 | TT |
| Preconditioning | Fascin homolog 1 | 6679745 | 3 | 3 | SCM |
| Preconditioning | HSP-70 (heat shock 70 kDa protein 5) | 25742763 | 2 | 2 | ISR |
| Preconditioning | HSP-60 (60 kDa heat shock protein) | 51702230 | 4 | 3 | ISR |
| Preconditioning | Inosine monophosphatase | 40018566 | 4 | 2 | Met |
| Preconditioning | MAP 1B (microtubule-associated protein 1B) | 1083716 | 3 | 3 | SCM |
| Preconditioning | MARCKS (myristoylated alanine-rich C-kinase substrate) | 27705710 | 2 | 2 | ST |
| Preconditioning | Na+/K+ ATPase β1 subunit | 6978549 | 2 | 2 | Met |
| Preconditioning | NCAM-1 (neural cell adhesion molecule 1) | 13928706 | 3 | 2 | Rec |
| Preconditioning | Phosphopyruvate hydratase | 109215 | 3 | 2 | Met |
| Preconditioning | Pigpen (rat homolog) | 34859348 | 2 | 2 | TT |
| Preconditioning | Plasma membrane calcium-transporting ATPase 1 | 14286099 | 5 | 3 | Met |
| Preconditioning | Protein disulfide-isomerase A6 precursor | 2501206 | 3 | 3 | Met |
| Preconditioning | Tubulin b5 protein | 38014544 | 5 | 3 | SCM |
| Preconditioning | Vimentin | 14389299 | 7 | 7 | SCM |
Proteins identified in P62 pull-down followed by mass spectrometry. Criteria for inclusion are listed in the Materials and Methods section, but include at least two unique peptide sequences for an identified protein. Proteins identified in both control and OGD are listed in the supplemental data (available at www.jneurosci.org as supplemental material). GI number, GenBank identification number; Met, metabolism; ISR, inflammatory stress response/defense proteins; ST, signal transduction/second messenger; SCM, sub-cell matrix and structural proteins; Rec, receptor; TT, transcription/translation.