Mutant | EC50 (μm) | nH | Imax (mA) | n |
---|---|---|---|---|
63-Phe (WT) | 113 ± 4 | 2.6 ± 0.2 | 8.6 ± 1.4 | 7 |
63-F1-Phe | 34.5 ± 0.1* | 2.8 ± 0.1 | 8.9 ± 0.7 | 7 |
63-F2-Phe | 210 ± 9* | 2.1 ± 0.2 | 3.2 ± 1.1* | 3 |
63-CN-Phe | 6.5 ± 0.4* | 2.7 ± 0.4 | 7.9 ± 1.5 | 5 |
63-F3-Phe | 348 ± 19* | 1.8 ± 0.2 | 4.2 ± 0.7* | 5 |
159-Phe (WT) | 109 ± 2 | 2.8 ± 0.2 | 7.4 ± 0.6 | 5 |
159-F1-Phe | 288 ± 6* | 3.1 ± 0.2 | 6.8 ± 1.1 | 4 |
159-F2-Phe | 11000 ± 200* | 2.0 ± 0.1* | 7.7 ± 0.6 | 6 |
159-CN-Phe | 46100 ± 1400* | 1.9 ± 0.1* | 9.2 ± 1.2 | 9 |
159-F3-Phe | 103000 ± 2700* | 2.3 ± 0.1 | 5.6 ± 0.6 | 7 |
207-Phe (WT) | 114 ± 3 | 2.2 ± 0.1 | 10.8 ± 1.1 | 4 |
207-ME-Phe | 1020 ± 20* | 2.2 ± 0.1 | 10.1 ± 3.3 | 4 |
207-O-ME-Phe | 2520 ± 140* | 2.2 ± 0.3 | 10.3 ± 0.4 | 4 |
207-F1-Phe | 151 ± 4 | 2.4 ± 0.1 | 9.5 ± 2.6 | 4 |
207-F2-Phe | 56700 ± 600* | 2.4 ± 0.1 | 8.1 ± 0.2* | 5 |
207-CN-Phe | 3910 ± 60* | 2.6 ± 0.1 | 9.7 ± 1.4 | 4 |
207-F3-Phe | 13500 ± 700* | 2.4 ± 0.2 | 8.4 ± 1.2 | 8 |
159,207-Phe (WT) | 131 ± 1 | 2.7 ± 0.1 | 9.3 ± 1.3 | 18 |
159,207-F1-Phe | 287 ± 10* | 2.6 ± 0.3 | 7.7 ± 1.7 | 13 |
Data are mean ± SEM.
↵*p < 0.05, significantly different from WT rescue (Student's t test).