Effects of 8-bromo-cGMP on tph-1::gfp expression in ADF
| Strains | 8-Bromo-cGMP (mm) | tph-1::gfp expression in ADF | |
|---|---|---|---|
| Very weak/invisible (N) | Medium/strong (N) | ||
| WT | 0 | 17 | |
| 1.25 | 0 | 40 | |
| 2.50 | 0 | 26 | |
| ocr-2(yz5) | 50 | 1 | |
| 1.25 | 70 | 10 | |
| 2.50 | 34 | 62 | |
| ocr-2(ak47) | 27 | 8 | |
| 1.25 | 15 | 14 | |
| 2.50 | 10 | 39 | |
Eggs from a strain were laid on NGM plates containing 8-bromo-cGMP at indicated concentrations and incubated at 20° C, and tph-1::gfp in hatched animals was analyzed 48 h later. The definitions of GFP levels are as follows: strong, WT levels as shown in Figure 2c; medium, the ADF cell body can be clearly recognized; very weak/invisible, ADF cell body is not discernible. N, Number of animals in each category examined. The results are based on one trial of ak47 and two independent trials of yz5.