Table 1.

Primer sequences used for real-time qPCR

GeneAccession numberForward sequenceReverse sequencePCR productAnnealing T°
ACCαNM1333605′-GCC TCT TCC TGA CAA ACG AG-3′5′-TGA CTG CCG AAA CAT CTC TG-3′23960
FASNM0079885′-TTG CTG GCA CTA CAG AAT GC-3′5′-AAC AGC CTC AGA GCG ACA AT-3′19260
SCD-1NM0091275′-CTT CAA GGG CAG TTC TGA GG-3′5′-CAA TGG TTT TCA TGG CAG TG-3′20360
LPLNM0085095′-CAG CTG GGC CTA ACT TTG AG-3′5′-AAT CAC ACG GAT GGC TTC TC-3′20660
CPT-1αAF0171745′-GTC GCT TCT TCA AGG TCT GG-3′5′-AAG AAA GCA GCA CGT TCG AT-3′23260
HSLBC0216425′-AGA CAC CAG CCA ACG GAT AC-3′5′-ATC ACC CTC GAA GAA GAG CA-3′23960
SREBP1cNM0114805′-TAG AGC ATA TCC CCC AGG TG-3′5′-GGT ACG GGC CAC AAG AAG TA-3′24560
β1-ARNM0074195′-CG GCC TTT CGT GTG TTT AAT-3′5′-GCA AAG GGA AAC ACT TCC TG-3′23260
β2-ARNM0074205′-GAG CAC AAA GCC CTC AAG AC-3′5′-GTT GAC GTA GCC CAA CCA GT-3′15360
β3-ARNM0134625′-GAC AGC CTC AAA TGC ATC CT-3′5′-CCC AGT CCA CAC ACC TTT CT-3′22060
NOR-1NM0157435′-TCA GCC TTT TTG GAG CTG TT-3′5′-TAA CCC ATG TCG CTC TGT GA-3′23460
HPRTNM0135565′-TGC TCG AGA TGT CAT GAA GG-3′5′-TAT GTC CCC CGT TGA CTG AT-3′19660