Summary of the genetic alterations in the rat Cav3.2 T-type calcium channel gene
| Mutation 1 | Mutation 2 | Mutation 3 | Mutation 4 | |
|---|---|---|---|---|
| Base pair number | 4751 | 2620 | 5439 | 6580 |
| Exon | 24 | 11 | 31 | 35 |
| Affected residue number | 1584 | 873 | 1813 | 2194 |
| R. norvegicus | G | A | C | T |
| NEC | G | G | T | G |
| GAERS | C | G | T | G |
| Codon change | CGG → CCGa | GCA → GCGb | TTC → TTTb | TCA → GCAb |
| Amino acid change | Arg → Pro | Ala → Ala | Phe → Phe | Ser → Ala |
| Type of mutation | Nonsynonymous | Synonymous | Synonymous | Nonsynonymous |
| Structural location | Linker III–IV | IIS3–IIS4 | IVS5 | COOH |
| Conservation between species | Conserved region | Conserved region | Conserved region | Nonconserved |
In addition to the gcm mutation, three more mutations were detected in the Wistar (NEC and GAERS) strains compared with R. norvegicus. Two of these mutations are silent and do not cause amino acid changes, whereas the third causes a TCA (serine) to CCA (alanine) change. However, none of these three mutations differed between the NEC and GAERS.
↵aCodon and amino acid change between NEC and GAERS.
↵bCodon and amino acid change between Wistar rats versus R. norvegicus.