Whole-cell conductance, steady-state inactivation, and recovery from inactivation properties of Cav3.2 (±25) splice variants in the presence and absence of the gcm
| Biophysical properties | Cav3.2 (+25) | Cav3.2 (+25) gcm | Cav3.2 (−25) | Cav3.2 (−25) gcm |
|---|---|---|---|---|
| Conductance | ||||
| V50 | −41.2 ± 1.2 | −43.3 ± 1.0 | −41.9 ± 1.2 | −42.6 ± 2.2 |
| k | −7.0 ± 0.3 | −6.0 ± 0.3 | −7.0 ± 0.4 | −7.0 ± 0.5 |
| Gmax | 7.7 ± 0.9 | 8.6 ± 1.0 | 9.7 ± 2.0 | 7.0 ± 1.06 |
| Peak I density (pA/pF) | −22.3 ± 3.2 | −30.1 ± 4.4 | −19.0 ± 3.5 | −19.4 ± 3.8 |
| Steady-state inactivation | ||||
| V50 | −65.1 ± 1.2 | −66.1 ± 1.2 | −65.2 ± 1.2 | −67.5 ± 2.3 |
| k | 3.9 ± 0.4 | 4.1 ± 0.3 | 3.9 ± 0.4 | 4.4 ± 1.0 |
| Recovery from inactivation | ||||
| τ 1 | 27.5 ± 2.1 | 24.1 ± 2.5 | 33.1 ± 3.8 | 25.3 ± 5.7 |
| τ 2 | 745.0 ± 32.2 | 436.8 ± 37.6* | 328.5 ± 35.8 | 430.5 ± 25.3** |
All values were calculated individually for each cell and the mean ± SEM taken to achieve the stated values (ANOVA; *p < 0.001, **p < 0.05 compared with wild-type control).