Table 1.

Period estimates and sample sizes for S6KII mutants and other genotypes

Genotypeat (h ± SEM)n
S6KIIign58-1/Y23.0  ± 0.3b72
S6KIIignP/Y (control)24.2  ± 0.256
w1118 (control)24.0  ± 0.241
S6KIIign58-1/Y, 20°C23.0  ± 0.07b81
w1118 (control), 20oC24.2  ± 0.05112
S6KIIign58-1/Y, 25°C23.3  ± 0.06b51
w1118 (control), 25°C24.3  ± 0.0559
S6KIIign58-1/Y, 29°C23.1  ± 0.06b87
w1118 (control), 29°C23.6  ± 0.0397
S6KIIign58-1/Y;S6KII5–5/+23.7  ± 0.04c67
w1118/Y;S6KII5–5/+ (control)23.7  ± 0.0530
w1118 (control)23.6  ± 0.0649
S6KIIign58-1/Y, 20°C22.9  ± 0.0771
S6KIIign58-1/Y, tim-UAS-Gal4/+, 20°C23.0  ± 0.0770
S6KIIign58-1/Y, UAS-S6KII/+, 20°C23.2  ± 0.0673
S6KIIign58-1/Y;tim-UAS-Gal4/+;UAS-S6KII/+, 20°C23.7  ± 0.04e90
S6KIIign58-1/Y;tim-Gal4/+;UAS-S6KII/+23.6  ± 0.05e78
S6KIIign58-1/Y;+/+;UAS-S6KII/+23.1  ± 0.0652
S6KIIign58-1/Y;pdf-Gal4/+;UAS-S6KII/+23.8  ± 0.0490
w1118 /Y;tim-UAS-Gal4/+;UAS-S6KII/+ (S6KII + overexpression)24.0  ± 0.08c18
w1118 (overexpression control)24.1  ± 0.0458
Andante/Y (control)25.1  ± 0.0840
Andante, S6KIIignP/Y (control)24.9  ± 0.0730
Andante, S6KIIign58-1/Y25.0  ± 0.1c37
S6KIIignP/Y;;Tik/+ (control)26.3  ± 0.0642
S6KIIign58-1/Y;;Tik/+26.1  ± 0.05c39
  • Bold indicates short-period phenotypes.

  • aActivity was monitored at 23°C in all experiments except those reported with different temperatures.

  • bDifferent from respective controls, p < 0.001.

  • cNot significantly different from control(s).

  • dSignificantly different from S6KIIign58-1/Y; +/+; UAS-S6KII/+ unrescued control, p < 0.001.

  • eSignificantly different from both unrescued controls, p < 0.001.