Primers used for real-time RT-PCR analyses
| Primer name | Sequence (5′–3′) | Tm (°C) | Concentration |
|---|---|---|---|
| GAPDH forward | AGC CTT CTC CAT GGT GGT GAA | 56–60 | 5 μm |
| GAPDH reverse | CGG AGT CAA CGG ATT TGG | 56–60 | 5 μm |
| CD3ϵ forward | AAG CAA GAG TGT GTC AGA ACT | 60 | 5 μm |
| CD3ϵ reverse | CTG ATT CAG GCC AGA ATA CAG | 60 | 5 μm |
| F4/80 forward | CAC GAC GGA GTT ACC CTT GT | 56 | 5 μm |
| F4/80 reverse | GCG AGG AAA AGG TAG TGC AG | 56 | 5 μm |
| GFAP forward | CTC ACC ATC CCG CAT CTC CAC AGT | 56 | 5 μm |
| GFAP reverse | GGA CAT CGA GAT CGC CAC CTA CAG | 56 | 5 μm |
| GluR1 forward | GGC ATC GGC TAC CAC TAC AT | 60 | 5 μm |
| GluR1 reverse | GTT CTT CCA CTG CTG CAT GA | 60 | 5 μm |
| GluR5 forward | CTG GCA GTG GCT CCT CTA AC | 60 | 5 μm |
| GluR5 reverse | GCT TCC GGT AGA GAA TGC TG | 60 | 5 μm |
| KA2 forward | TGG GGA TCA GCA TCC TCT AC | 60 | 5 μm |
| KA2 reverse | GCG AGG TAG GCG AGA AGC ATG AA | 60 | 5 μm |