Primary antibodies used for immunocytochemistry
| Antigen | Host species | Dilution | Sources |
|---|---|---|---|
| β3-tubulin | Rabbit | 1:1000 | Covance; PRB-435P |
| B-FABP | Rabbit | 1:1000 | Gift from T. Müller (Max Delbrück Center for Molecular Medicine, Berlin, Germany) |
| BrdU (BU-1) | Mouse IgG2a | 1:100 | GE Healthcare; RPN202 |
| BrdU [BU1/75 (ICR1)] | Rat IgG2a | 1:50 | AbD Serotec; MCA2060 |
| CREB (48H2) | Rabbit | 1:400 | Cell Signaling; 9197 |
| Phospho-CREB (87G3) | Rabbit | 1:25 | Cell Signaling; 9198 |
| Doublecortin (C-18) | Goat | 1:50 | Santa Cruz; sc-8066 |
| GFAP | Chicken IgY | 1:2000 | Neuromics; CH22102 |
| HuC/D | Mouse-biotin | 1:50 | Invitrogen; A21272 |
| HuD (N-15) | Goat | 1:100 | Santa Cruz; sc-5977 |
| Ki67 (SP6) | Rabbit | 1:80 | Vector Laboratories; VP-RM04 |
| LC3B | Rabbit | 1:800 | Novus; NB600-1384 |
| LC3B | Rabbit | 1:1000 (WB) | Sigma-Aldrich; L7543 |
| Musashi-1 | Rabbit | 1:100, 1:1000 (WB) | Neuromics; RA14128 |
| Nestin | Rabbit | 1:100 | Gift from R. D. Goldman (Department of Cell and Molecular Biology, Northwestern University, Chicago, IL) |
| Neurofilament-H | Chicken IgY | 1:2000 | Neuromics; CH22104 |
| nNOS | Rabbit | 1:1000 | Santa Cruz; sc-648 |
| p75NTR | Rabbit | 1:500 | Gift from M. V. Chao (Skirball Institute of Biomolecular Medicine, New York University, New York, NY) |
| Peripherin (C-19) | Goat | 1:1000 | Santa Cruz; sc-7604 |
| PGP9.5 | Rabbit | 1:1000 | AbD Serotec; 7863-0504 |
| Phox2b | Rabbit | 1:500 | Gift from J. F. Brunet (École Normale Supérieure, Département de Biologie, Paris, France) |
| S100β | Rabbit | 1:1000 | Neuromics; RA25022 |
| Sox10 | Guinea pig | 1:500 | Gift from M. Wegner (Institut für Biochemie, Universität Erlangen-Nürnberg, Erlangen, Germany) |
| Synaptotagmin | Rabbit | 1:1000 | Sigma-Aldrich; S2177 |
| α-Tubulin (DM1A) | Mouse IgG1 | 1:4000 (WB) | Millipore; 05-829 |
WB, Western blotting.