Summary of single channel properties
| Parameters | NR2A | NR2A-ΔATD | NR2A-(2D-ATD) | NR2D | NR2D-ΔATD | NR2D-(2A-ATD) |
|---|---|---|---|---|---|---|
| N | 4 | 4 | 5 | 6 | 3 | 4 |
| POPEN | 0.48 ± 0.05 | 0.006 ± 0.003a | 0.035 ± 0.017a | 0.012 ± 0.002 | 0.065 ± 0.015a | 0.059 ± 0.011a |
| Open duration (ms) | 1.7 ± 0.20 | 1.6 ± 0.33 | 1.1 ± 0.08a | 0.61 ± 0.04 | 0.86 ± 0.08a | 0.99 ± 0.04a |
| τ1, ms (%) | 0.1 (9%) | 0.25 (38%) | 0.1 (9%) | 0.1 (21%) | 0.12 (1%) | 0.10 (1%) |
| τ2, ms (%) | 1.7 (91%) | 2.8 (62%) | 1.1 (91%) | 0.61 (79%) | 0.86 (99%) | 0.99 (99%) |
| Shut duration (ms) | 1.9 ± 0.45 | 360 ± 86a | 69 ± 21a | 60 ± 13 | 14 ± 2.6a | 17 ± 4.0a |
| τ0, ms (%) | 0.02 (49%) | 0.02 (53%) | 0.02 (41%) | 0.027 (30%) | 0.029 (33%) | 0.023 (48%) |
| τ1, ms (%) | 0.20 (16%) | 0.36 (26%) | 0.25 (15%) | 0.28 (14%) | 0.20 (11%) | 0.26 (23%) |
| τ2, ms (%) | 0.93 (27%) | 2.9 (7%) | 1.3 (13%) | 13 (20%) | 1.3 (11%) | 1.9 (9%) |
| τ3, ms (%) | 2.8 (8%) | 17 (7%) | 13 (26%) | 66 (31%) | 12 (35%) | 14 (13%) |
| τ4, ms (%) | 540 (<1%) | 61 (3%) | 85 (3%) | 260 (5%) | 46 (10%) | 76 (7%) |
| τ5, ms (%) | – | 4400 (4%) | 3200 (2%) | 16000 (<1%) | 500 (<1%) | 1300 (<1%) |
| Amplitude 1 (pA) | 5.5 ± 0.04 | 5.3 ± 0.06 | 5.5 ± 0.11 | 4.3 ± 0.6 | 4.3 ± 0.26 | 4.7 ± 0.21 |
| Amplitude 2 (pA) | – | – | 4.4 ± 0.07 | 2.5 ± 0.3 | 2.6 ± 0.11 | 2.9 ± 0.12 |
| γ1, ps (%) | 69 (100%) | 66 (100%) | 69 (88%) | 53 (70%) | 54 (70%) | 58 (66%) |
| γ2, ps (%) | –b | –b | 55 (12%) | 31 (30%) | 33 (30%) | 36 (34%) |
| H→ L (%) | – | – | – | 64 | 60 | 58 |
| L→H (%) | – | – | – | 36 | 40 | 42 |
Values are shown with two significant digits as mean or mean ± SEM; n is the number of single channel patches from HEK 293 cells used to generate the data. Component values of open periods and shut durations and the transitions between high (H) and low (L) conductance levels are from the averages of the fittings from each of the single channel patches.
↵aFor mean open probability, open duration, shut duration, and unitary current amplitude, p < 0.01, one-way ANOVA with Bonferroni's post hoc test, compared with the corresponding wild type.
↵bA minority of patches from NR2A- and NR2A-ΔATD-transfected cells show a subconductance level with percentage less than 5%.