PSD-95 | α1α3 | Apposed | Nonlabeled | Total | |
---|---|---|---|---|---|
Quantification on dendritic shafts | |||||
4 dpi | 0.21 ± 0.02 ^{††} | 0.15 ± 0.02 | 0.10 ± 0.01 | ||
5 dpi | 0.20 ± 0.02 ^{§} | 0.10 ± 0.01 ^{‡} | 0.22 ± 0.03^{**} | ||
7 dpi | 0.15 ± 0.01^{ §§ † } | 0.07 ± 0.01^{°} ^{‡} | 0.21 ± 0.02^{°°} | ||
Quantification on spines | |||||
4 dpi | 0.04 ± 0.01 ^{§} | 0.007 ± 0.003 | 0.03 ± 0.007 | 0.03 ± 0.006 | 0.1 ± 0.02 |
5 dpi | 0.04 ± 0.01 ^{†} | 0.01 ± 0.004 ^{‡} | 0.10 ± 0.01^{**} | 0.03 ± 0.007 ^{¤} | 0.15 ± 0.02 |
7 dpi | 0.07 ± 0.01 ^{§§§} | 0.007 ± 0.003 ^{‡} | 0.10 ± 0.02^{°°} | 0.01 ± 0.003^{ ¥ ¤¤ } | 0.19 ± 0.02 |
Clusters were distinguished based on whether PSD-95 or α1/α3 subunit were either alone or apposed to each other. At the top (“Quantification on dendritic shafts”), the density of clusters formed on the shaft (mean ± SD) is given per micrometer/dendrite length. At the bottom (“Quantification on spines”), the density of spines (mean ± SD) per micrometer/dendrite length is given based on the type of cluster apposed to the spine. The density of unlabeled spines and the total spine density are indicated in the last two columns. The symbols indicate the results of Bonferroni's post hoc tests (for main statistical analysis, see Results).
↵**5 dpi different from 4 dpi, p < 0.001;
↵°7 dpi different from 4 dpi, p < 0.05;
↵°°p < 0.001;
↵ ^{†}PSD-95 different from apposed, p < 0.05;
↵ ^{††} p < 0.001;
↵ ^{‡}α1α3 different from apposed, p < 0.001;
↵ ^{§}PSD-95 different from α1α3, p < 0.05;
↵ ^{§§} p < 0.01;
↵ ^{§§§} p < 0.001;
↵ ^{¥}nonlabeled different from PSD-95, p < 0.001;
↵ ^{¤}nonlabeled different from apposed, p < 0.01;
↵ ^{¤¤} p < 0.001.