Cell-type composition of retinas from WT, Prom-1+/−, and Prom-1−/− at two different time points during postnatal development as determined by immunostaining for cell-type-specific markers
| Marker | Cell type | P10 | P20 | ||||
|---|---|---|---|---|---|---|---|
| WT | +/− | −/− | WT | +/− | −/− | ||
| Recoverin | Rods and cones | 62.2 ± 5.3 | 68.7 ± 8.1 | 66.1 ± 7.1 | 74.1 ± 7.4 | 70.2 ± 7.3 | 43.2 ± 6.4 |
| Rhodopsin | Rods | 43.4 ± 5.1 | 40.3 ± 3.9 | 41.4 ± 2.8 | 58.9 ± 5.1 | 54.4 ± 7.7 | 24.4 ± 4.5 |
| PNA | Cones | 1.16 ± 0.2 | 1.21 ± 0.2 | 1.01 ± 0.2 | 1.47 ± 0.3 | 1.42 ± 0.3 | 0.98 ± 0.2 |
| Calbindin | Horizontal cells | 2.28 ± 0.3 | 2.48 ± 0.3 | 2.08 ± 0.5 | 3.85 ± 0.7 | 3.48 ± 0.5 | 3.22 ± 0.6 |
| Calretinin | Amacrine cells | 3.43 ± 0.7 | 2.95 ± 0.8 | 3.28 ± 0.8 | 5.27 ± 0.7 | 4.99 ± 0.9 | 5.96 ± 1.0 |
| PKC-α | Bipolar cells | 24.3 ± 5.6 | 26.4 ± 1.1 | 21.9 ± 1.7 | 42.0 ± 6.9 | 39.4 ± 9.7 | 35.5 ± 3.3 |
| NeuN | RGCs | 3.95 ± 0.2 | 4.14 ± 0.2 | 3.89 ± 0.2 | 4.85 ± 0.9 | 4.40 ± 0.4 | 4.80 ± 0.5 |
| GFAP | Müller glia | 0.90 ± 0.2 | 1.11 ± 0.3 | 1.09 ± 0.3 | 1.46 ± 0.2 | 1.69 ± 0.9 | 14.2 ± 2.5 |
RGCs, Retinal ganglion cells. Bold indicates statistical significance versus WT (p < 0.02).