Table 1.

Distribution of BDNF protein in the medulla oblongata of P35 Mecp2 wild-type mice and comparison to P35 Mecp2-null mice

StructureP35 Mecp2 wild typeP35 Mecp2 null versus wild type
BDNF-IR cell bodiesBDNF-IR processesBDNF-IR cell bodiesBDNF-IR processes
Intermediate reticular nucleus++↓↓↓
Gracile nucleus+
Lateral reticular nucleus++
Spinal trigeminal tract++
Spinal trigeminal nucleus, caudal/interpolar+++++
Inferior olive+++++↓↓
Nucleus tractus solitarius++++↓↓↓
Motor nucleus of the glossopharyngeal nerve+
Motor nucleus of the vagus nerve++++↓↓↓
Area postrema+++++↓↓↓↓↓
A1/C1++↓↓
Raphe obscurus+
Nucleus ambiguus++↓↓
Rostroventrolateral reticular nucleus++↓↓↓
Ventral spinocerebellar tract++
Parvocellular reticular nucleus++
Prepositus nucleus+++/++↓↓
Raphe magnus++
Lateral paragigantocellularis nucleus++↓↓
Principal sensory nucleus of the trigeminal nerve, dorsomedial+++
Principal sensory nucleus of the trigeminal nerve, ventrolateral++
  • For BDNF-immunoreactive (IR) cell bodies: −, no cells observed; +, occasional cells; ++, scattered cells; +++, densely packed cells. For BDNF-IR cell processes: −, no processes observed; +, low density; ++, moderate density; +++, high density; ++++, very high density. For changes in null mice compared to wild types: −, no obvious change; ↓, mild decrease; ↓↓, moderate decrease; ↓↓↓, sharp decrease. Data was collected on three wild-type and three Mecp2-null mice.