Effect of Lfc suppression on neuronal shape parameters
| Groups | Number of axons per cell | Axonal length per cell | Number of axonal branches | Length of minor processes | Number of minor processes |
|---|---|---|---|---|---|
| SC-sh-HcRed | 1.36 ± 0.11 | 589 ± 43 | 11 ± 1.8 | 36 ± 1.8 | 4.96 ± 0.42 |
| sh1-Lfc-HcRed | 2.40 ± 0.18* | 1070 ± 48* | 32 ± 2.6* | 42 ± 1.3 | 6.10 ± 0.52 |
All measurements were performed at 3 d in vitro. Neurons were transfected at 2 d in vitro and fixed 24 h later. Two micrograms of plasmid were used for this experiment. Each value represents the mean ± SEM of at least 30 cells for each experimental condition. Statistical analysis was determined by one-way ANOVA, followed by a Tukey's honestly significant difference post hoc test to compare SC-sh-HcRed- versus sh1-Lfc-HcRed-transfected neurons (*p < 0.0002).