Table 1.

Effect of Lfc suppression on neuronal shape parameters

GroupsNumber of axons per cellAxonal length per cellNumber of axonal branchesLength of minor processesNumber of minor processes
SC-sh-HcRed1.36 ± 0.11589 ± 4311 ± 1.836 ± 1.84.96 ± 0.42
sh1-Lfc-HcRed2.40 ± 0.18*1070 ± 48*32 ± 2.6*42 ± 1.36.10 ± 0.52
  • All measurements were performed at 3 d in vitro. Neurons were transfected at 2 d in vitro and fixed 24 h later. Two micrograms of plasmid were used for this experiment. Each value represents the mean ± SEM of at least 30 cells for each experimental condition. Statistical analysis was determined by one-way ANOVA, followed by a Tukey's honestly significant difference post hoc test to compare SC-sh-HcRed- versus sh1-Lfc-HcRed-transfected neurons (*p < 0.0002).