Table 1.

Summary of colabeling of GFP+ SNPs and RGCs with Ki67, Pax6, Tbr2, and TUJ1

Percentage of GFP+ cells colabeled with
Ki67Pax6Tbr2TUJ1
RGCs11.73 ± 2.4115.66 ± 5.647.98 ± 1.402.96 ± 0.84
SNPs7.75 ± 0.8317.60 ± 0.694.78 ± 0.639.65 ± 1.39
  • Immunostaining was performed on tissue harvested 24 h after electroporation at E14.5 with either pTα1-hGFP or pGLAST-eGFPf. Cell counts were performed on 40× collapsed Z-stacks, across the entire width of the image (225 μm) and up to 200 μm from the ventricular surface. Values represent the percentage of GFP+ cells coexpressing each protein or transcription factor ± SEM; n = 4 per group.