The relative free energy of Na+ selectivity of the binding site in the C terminus of Kir3.1N217D and wild-type Slo2.2
| ΔΔGNa+/K+ | ΔΔGNa+/Li+ | |
|---|---|---|
| Kir3.1 | 2.9 | 1.1 |
| Slo2.2 | 2.5 | 0.8 |
| D818R | N/A | N/A |
| D818E | 1.9 | −0.2 |
| D818N | 1.1 | 4.9 |
All units are in kilocalories per moles. The positive sign in the relative free energy (ΔΔG) refers to site preferences for Na+.