Table 1.

Effect of DEAB treatments at different times on otolith size, number of neuromasts of the primary and secondary systems, and number of neurons in the PLL ganglion

t (hpf)OtolithsNMINMII/DN1NeuronsN2
5–73.8 ± 0.3ndnd10nd
6–81.9 ± 0.87.3 ± 1.23.8 ± 0.845nd
7–91.0 ± 0.55.2 ± 1.73.1 ± 0.74110.1 ± 1.914
8–101.6 ± 1.14.1 ± 1.92.6 ± 0.9406.6 ± 2.424
9–113.7 ± 0.46.3 ± 1.33.7 ± 0.83012.9 ± 1.512
ctrl4.09.1 ± 0.74.4 ± 0.52019.8 ± 2.212
  • N1, Number of samples for the first three columns, examined at 4 dpf; N2, number of samples for the neuronal counts, examined at 24 hpf. The cumulated size of otoliths was measured as the sum of the heights of the otoliths present, and the number and type of neuromasts were determined after alkaline phosphatase labeling (see Materials and Methods).