Table 1.

Primary antibodies used in this study

AntibodyImmunogenHostDilutionApplicationSource
R1736Residues 595-611 of APP695 (αAPPs)Rabbit1:1000WBD. Selkoe (Center for Neurologic Diseases, Boston, MA)
R12821-40Rabbit1:1000IHCD. Selkoe
C8Against last 20 C-terminal residues of APPRabbit1:1000WBD. Selkoe
IDE-1Residues 62-73 of human IDE-1Rabbit1:1000WBD. Selkoe
PKCαC terminus of PKCα of human originMouse1:1000WBSanta Cruz Biotechnology
ADAM10Residues 732-748 of human ADAM10Rabbit1:200WBSigma
TACEC terminal of human TACERabbit1:200WBProSci
CD45Mouse B-cellsRat1:5000IHCSerotec
GFAPBovine GFAPRabbit1:1000IHCDako
AT8Phospho-Ser202/Thr205Mouse1:25IFPierce
AT180Phospho-Thr231/Ser235Mouse1:50IFPierce
PHF-1Phospho-Ser396/Ser404Mouse1:100IFP. Davies (Albert Einstein College of Medicine, Bronx, NY)
  • IHC, Immunohistochemistry; IF, immunofluorescent staining; WB, Western blot.