Table 1.

Efficiency of neuronal induction by NeuroD1, Neurog1, and Sox2 at different ages

cDNATotal number of explants (T); totalnumber of transfected cells (N)TuJ1-positive cells(% of total)
Control.EGFP (E13.5)T = 4; N = 1661 (0.6%)
NeuroD1.EGFP (E13.5)T = 5; N = 156114 (73%)
NeuroD1.EGFP (E16.5)T = 4; N = 16067 (41%)
NeuroD1.EGFP (P1)T = 3; N = 13033 (25%)
Neurog1.EGFP (E13.5)T = 4; N = 11129 (26%)
Neurog1.EGFP (E16.5)T = 5; N = 25340 (15%)
Neurog1.EGFP (P1)T = 7; N = 14311 (7%)
Sox2.nucEGFP (E13.5)T = 6; N = 505199 (39%)
Sox2.nucEGFP (E16.5)T = 4; N = 52682 (16%)
Sox2.nucEGFP (P1)T = 5; N = 33811 (3%)
  • Nonsensory cells located in either Kolliker's organ or the lesser epithelial ridge that were transfected with the indicated constructs were identified based on expression of GFP. Neuronal identity was established based on expression of TuJ1.