Table 1.

Quantification of cell proliferation in the hypothalamus of adult AgRP-Tfam mutant mice

ARCVMHDMHLHA
CountMean ± SEMn, NCountMean ± SEMn, NCountMean ± SEMn, NCountMean ± SEMn, N
Ki-67
    Control281.8 ± 0.416, 5141.0 ± 0.313, 590.7 ± 0.213, 5141.0 ± 0.213, 5
    Mutant1535.5 ± 0.7**28, 6281.2 ± 0.323, 6160.7 ± 0.223, 6150.6 ± 0.223, 6
PCNA
    Control444.0 ± 1.211, 5495.5 ± 2.49, 4262.9 ± 0.79, 4313.4 ± 1.19, 4
    Mutant19511.5 ± 1.1**17, 6855.7 ± 1.315, 6573.6 ± 0.616, 6412.6 ± 0.616, 6
BrdU
    Control1143.9 ± 0.429, 4624.5 ± 0.814, 4362.6 ± 0.614, 4241.9 ± 0.413, 4
    Mutant3329.8 ± 1.0**34, 4794.9 ± 0.616, 4603.8 ± 0.516, 4332.1 ± 0.416, 4
  • Number of cells positive for Ki-67, PCNA, or BrdU was quantified in different regions of the hypothalamus. Cell counts were corrected using the Abercrombie correction method (Abercrombie, 1946). The Count columns contain the total number of positive cells corrected using the Abercrombie method. Mean is the average number of positive cells per section. All sections were 10 μm. n, Number of sections evaluated; N, number of mice from which the sections were evaluated. **p ≤ 0.01, the difference between mutant and control mice.