Table 1.

Complete survey of the rat SV Rabome

Rab isoformGi numberQuantified byWB ratio (±SD)iTRAQ ratio (±SD)Localization byGDI Pool IP/MS detection
S3/HLP2/HSV/HS3/HLP2/HSV/HEGFPABSB labeling
Rab1agi|45433570MS & WB0.47 (0.33)2.86 (0.52)3.60 (0.61)0.12 (0.29)2.87 (0.19)5.29 (0.63)YesYesNoYes
Rab2agi|13929006MS & WB0.34 (0.08)2.93 (0.04)3.79 (0.41)0.39 (0.05)2.81 (0.22)4.93 (0.96)N/AYesNoYes
Rab2bgi|83415090MS0.34 (0.08)2.93 (0.04)3.79 (0.41)0.36 (N/A)1.04 (N/A)1.06 (N/A)N/AYesNoYes
Rab3agi|61098195MS & WB2.43 (0.32)7.66 (0.16)12.42 (0.72)0.38 (0.04)6.11 (1.52)13.07 (1.40)YesYesStrongYes
Rab3bgi|13592037MS & WB1.05 (0.28)4.98 (0.32)10.53 (0.73)0.41 (0.01)4.23 (1.38)6.62 (0.56)N/AYesStrongYes
Rab3cgi|19424194MS & WB0.45 (0.02)6.30 (0.82)9.89 (1.85)0.30 (0.01)6.80 (0.31)19.04 (1.84)YesN/AStrongYes
Rab4agi|77404180WB1.30 (0.03)1.80 (0.89)5.01 (0.24)N/AN/AN/AN/AN/AN/AYes
Rab4b*gi|8394136MS & WB1.30 (0.03)1.80 (0.89)5.01 (0.24)0.05 (N/A)0.90 (N/A)1.84 (N/A)YesN/AWeakYes
Rab5agi|12083645MS & WB1.24 (0.03)2.63 (0.60)6.91 (0.27)0.04 (0.25)2.18 (0.19)5.30 (1.35)YesYesModerateYes
Rab5bgi|121583768MS & WB1.24 (0.53)2.63 (0.60)6.91 (0.27)0.35 (0.18)2.79 (0.70)5.68 (0.20)N/AYesModerateYes
Rab5cgi|157786692MS & WB1.24 (0.53)2.63 (0.60)6.91 (0.27)N/A1.51 (N/A)3.30 (N/A)N/AYesModerateYes
Rab6agi|213972608MS & WB1.54 (0.36)1.35 (0.48)1.68 (0.42)0.73 (N/A)2.58 (N/A)2.59 (N/A)N/AYesNoYes
Rab6bgi|157817539MS & WB1.54 (0.36)1.35 (0.48)1.68 (0.42)0.54 (N/A)2.28 (N/A)3.37 (N/A)YesYesNoYes
Rab7agi|13027392MS & WB1.06 (0.87)1.11 (0.27)4.47 (0.67)2.29 (0.05)2.13 (0.24)3.71 (0.16)YesN/ANoYes
Rab8bgi|23463313MSN/AN/AN/AN/A1.59 (N/A)1.43 (N/A)YesN/ANoYes
Rab10gi|61889071MS & WB0.34 (0.44)3.36 (0.62)6.30 (0.3)0.28 (0.01)2.81 (0.38)6.33 (0.09)YesN/AModerateYes
Rab11bgi|14249144MS & WB0.76 (0.07)3.28 (0.02)4.67 (0.20)0.46 (0.22)3.01 (0.15)4.28 (0.63)YesN/AModerateYes
Rab12*gi|158186685N/AN/AN/AN/AN/AN/AYesN/ANoYes
Rab14gi|16758368MS & WB0.74 (0.17)3.74 (0.08)6.94 (0.42)0.52 (0.02)3.99 (0.58)9.95 (0.75)YesN/AModerateYes
Rab15gi|38454238MSN/AN/AN/AN/A2.79 (0.32)3.76 (0.26)N/AN/AN/ANo
Rab18gi|60223069MSN/AN/AN/A0.12 (0.38)2.94 (0.17)2.05 (0.06)YesN/ANoYes
Rab21gi|51948448MSN/AN/AN/AN/A3.22 (0.94)2.64 (0.55)N/AN/AN/AYes
Rab26*gi|19424272MSN/AN/AN/AN/AN/AN/AN/AN/AN/AYes
Rab27bgi|16758202WB0.22 (0.29)4.03 (0.65)9.95 (0.31)N/AN/AN/AYesYesStrongNo
Rab33bgi|157822117MS & WB1.33 (0.57)2.10 (0.44)3.93 (1.06)0.36 (N/A)1.04 (N/A)1.06 (N/A)YesN/ANoYes
Rab34/39#gi|58865850/gi|157822741MS & WB0.442.45 (0.81)3.98 (0.63)N/AN/AN/AN/AN/AN/ANo
Rab35gi|61556789MS & WB0.17 (0.06)2.61 (0.34)4.34 (0.08)0.63 (N/A)1.85 (N/A)1.96 (N/A)YesYesNoYes
Rab43gi|66730539N/AN/AN/AN/AN/AN/AN/AN/AN/ANo
  • *Peptide redundancy. Rab4b: 1a, 3a–c, 4a, 4b, 8b, 10, 12; Rab12: 1a, 3a–b, 4a, 8a, 10, 14, 35; Rab26: 1a, 3a–b, 4a, 8a, 10, 14, 35.

  • #Unique peptide poor quality for quantitation. Rab34/39.

  • Note that localization studies were performed using EGFP-fused Rabs and/or Rab-specific antibodies (AB). SB labeling was scored weak-to-strong based on Rab colocalization and correlation with synaptophysin immunostaining. N/A, Not applicable; WB, Western blotting.