Table 1.

Numbers of mice used in behavioral studies

FigureTreatment in miceSerotonergic dose and n values
1A,B5-HTP (mg/kg, i.p.) ±M100100150200M100 + 200
WT, βarr2-KOn = 13, 8n = 7, 5n = 8, 7n = 6, 6
1C,D5-HT (μg, i.c.v.) ±M100102040M100 + 40
WT, βarr2-KOn = 4, 5n = 7, 5n = 5, 6n = 4, 4
1E,FVeh/Clor (±M100) + 5-HTPVeh +50Veh +100Clor +50Clor +100Clor + M100 +100
WT, βarr2-KOn = 6, 5n = 6, 5n = 5, 6n = 9, 8n = 4, 4
2A,BVeh/MTZ + 5-HTPVeh + 200MTZ + 200
WT, βarr2-KOn = 4, 5n = 4, 5
3A,BN-Me-5-HT (μg, i.c.v.)102040
WT, βarr2-KOn = 4, 4n = 5, 5n = 4, 4
3CVeh/M100 + N-Me-5-HTVeh + 20M100 + 20
WT, βarr2-KOn = 4, 4n = 4, 4
3DVeh/MTZ + N-Me-5-HTVeh + 20MTZ + 20
WTn = 5n = 4
3E,F5-MeO-DMT (mg/kg, i.p.)51015
WT, βarr2-KOn = 9, 10n = 10, 16n = 5, 5
3GVeh/M100 + 5-MeO-DMTVeh + 10M100 + 10
WT, βarr2-KOn = 5, 9n = 5, 5
3HVeh/MTZ + 5-MeO-DMTVeh + 10MTZ + 10
C57BL/6n = 6n = 4
6A,BVeh, LY, PP2, AKTi + 5-HTPVeh + 200LY + 200PP2 + 200AKTi + 200
C57BL/6n = 10n = 5n = 5n = 5
6CVeh, AKTi + 5-MeO-DMT10AKTi + 200
C57BL/6n = 6n = 4
7A,BVeh, LY, PP2, AKTi + 5-HTP200LY + 200PP2 + 200AKTi + 200
βbarr2-KOn = 7n = 6n = 6n = 6
8A,BVeh, AKTi, MTZ, AKTi + MTZ + 5-HTP200MTZ + 200AKTi + 200MTZ + AKTi +200
WTn = 11n = 6n = 5n = 5
  • Veh, Vehicle; 5-HT, serotonin; M100, M100907; Clor, clorgyline; N-Me-5-HT, N-methylserotonin; LY, LY294002. Serotonergic dosing routes: 5-HTP, mg/kg, i.p.; 5-HT and N-methylserotonin, μg, i.c.v.; 5-MeO-DMT, mg/kg, i.p. For inhibitor and antagonist dosage information, see Materials and Methods.