Table 1.

Normal GC proliferation, differentiation, cell death, and blood vessel density in VEGF∂/∂ and VEGF188/188 mice

WTVEGF∂/∂VEGF188/188p value
P27+ GCs—lower EGL (P10) (p27+ GCs/100 μm EGL)38.5 ± 3.055.3 ± 5.6p < 0.05
Pax6 density (P10) (Pax6+ GCs/103 μm2 EGL)26.1 ± 4.127.6 ± 3.4p = NS
GCP proliferation—upper EGL (P10) (Ki67+ GCs/100 μm EGL)33.6 ± 3.037.8 ± 1.5p = NS
GCP proliferation (P10) (BrdU+ GCs/100 μm EGL)26.5 ± 1.523.3 ± 1.3p = NS
GCP proliferation (P10) (PH3+ GCs/mm EGL length)13.2 ± 1.414.5 ± 2.2p = NS
GC apoptosis (P10) (act-casp-3+ GCs/105 μm2 EGL)10.9 ± 0.610.9 ± 0.9p = NS
Blood vessel density (P12) (CD31+ area, as % of total cerebellum area)5.7 ± 0.84.8 ± 0.8p = NS
P27+ GCs—lower EGL (P12) (p27+ GCs/100 μm EGL)26.2 ± 1.617.6 ± 2.0p < 0.05
Pax6 density (P12) (Pax6+ GCs/103 μm2 EGL)29.7 ± 0.727.1 ± 1.3p = NS
GCP proliferation—upper EGL (P12) (Ki67+ GCs/100 μm EGL)12.0 ± 1.412.0 ± 1.0p = NS
GCP proliferation (P10) (BrdU+ GCs/100 μm EGL area)19.4 ± 0.519.3 ± 1.7p = NS
GC proliferation (P10) (PH3+ GCs/mm EGL length)17.8 ± 2.518.8 ± 1.4p = NS
GC apoptosis (P10) (act-casp-3+ GCs/105 μm2 EGL)10.6 ± 0.810.6 ± 0.7p = NS
Blood vessel density (P12) (CD31+ area, as % of total area)4.2 ± 0.64.1 ± 0.6p = NS