Effects of PARP-1 activation on glycolytic intermediates upstream of GAPDH
| Condition | DHAP (pmol/mg) | GAP (pmol/mg) | FBP (pmol/mg) |
|---|---|---|---|
| Control | 2.24 ± 0.74 | 0.93 ± 0.26 | 1.64 ± 0.97 |
| MNNG | 11.18 ± 2.57# | 2.37 ± 0.62# | 8.52 ± 1.69# |
| MNNG + PJ34 | 2.28 ± 0.96** | 1.06 ± 0.23* | 2.22 ± 1.29** |
| MNNG + DPQ | 2.58 ± 0.84** | 1.20 ± 0.34 | 3.19 ± 1.15** |
| MNNG + NAD | 1.52 ± 0.90** | 1.13 ± 0.22* | 3.39 ± 1.29** |
| IA | 9.83 ± 2.06# | 3.10 ± 1.14# | 6.93 ± 0.73# |
Mouse cortical neurons were exposed to 75 μm MNNG for 30 min. PJ34 (200 nm) and DPQ (25 μm) were added with MNNG, and NAD+ (5 mm) was added after MNNG washout. Levels of DHAP, GAP, and FBP were measured at 30 min after MNNG washout. Iodoacetate (IA) (250 μm) was used as a positive control for GAPDH inhibition. Data are means ± SEM (n = 3).
↵#p < 0.01 versus control; *p < 0.05, **p < 0.01 versus MNNG.