Effects of α-syn monomer and oligomers extracted from different brain regions of A53T and WT α-syn transgenic mice on the kinetics of recombinant α-syn fibril formation in vitro
| Brain region/fraction | RFU (ThioT) |
|---|---|
| α-syn alone | 181.1 ± 9.8 |
| α-syn + A53T SC 53Å oligomer | 223.5 ± 8.7* |
| α-syn + A53T SC monomer | 159.0 ± 10.7 |
| α-syn + A53T OB 53Å oligomer | 136.8 ± 11.3* |
| α-syn + A53T OB monomer | 156.8 ± 18.1 |
| α-syn + WT SC 53Å fraction | 187.4 ± 11.3 |
| α-syn + WT OB 53Å fraction | 182.8 ± 5.3 |
The values represent the mean ± SEM (n = 4–6). Fluorescence intensity was recorded after 24 h incubation.
↵*p < 0.05, Student's t test analysis compared with purified recombinant α-syn protein alone. RFU, Relative fluorescence units.