Summary of microarray and qPCR data from the acute cocaine experiment
| Gene symbol | Description | Gene ID | Microarray | Quantitative PCR |
|---|---|---|---|---|
| Fos | c-fos; FBJ osteosarcoma oncogene; Immediate early gene | 314322 | +2.7-fold | F(2,9) = 1.887, p = 0.207 |
| β-gal + vs β-gal−: p = 0.229 | ||||
| β-gal+ vs NeuN+: p = 0.09 | ||||
| β-gal− vs NeuN+: p = 0.446 | ||||
| FosB | fra-2; FBJ osteosarcoma oncogene B; Immediate early gene | 308411 | — | F(2,11) = 5.520, p < 0.05 |
| β-gal+ vs β-gal−: +6.3-fold; n = 5, 6; p < 0.05 | ||||
| β-gal+ vs NeuN+: +7.5-fold; n = 5, 3; p < 0.05 | ||||
| β-gal− vs NeuN+: p = 0.938 | ||||
| Arc | Activity-regulated cytoskeleton-associated protein | 54323 | +4.6-fold | F(2,8) = 17.402, p < 0.005 |
| β-gal+ vs β-gal−: +6.6-fold; n = 4, 4; p < 0.005 | ||||
| β-gal+ vs NeuN+: +3.4-fold; n = 4, 3; p < 0.005 | ||||
| β-gal− vs NeuN+: p = 0.419 | ||||
| Nr4a3 | Nuclear receptor subfamily 4, group A, member 3; transcription factor | 58853 | +5.2-fold | F(2,9) = 11.456, p < 0.005 |
| β-gal+ vs β-gal−: +8.0-fold; n = 4, 5; p < 0.005 | ||||
| β-gal+ vs NeuN+: +5.1-fold; n = 4, 3; p < 0.01 | ||||
| β-gal− vs NeuN+: p = 0.742 | ||||
| Pdyn | Prodynorphin; peptide found in D1-expressing striatal neurons | 29190 | +2.3-fold | F(2,6) = 70.047, p < 0.001 |
| β-gal+ vs β-gal−: +3.6-fold; n = 3, 3; p < 0.001 | ||||
| β-gal+ vs NeuN+: +3.1-fold; n = 3, 3; p < 0.001 | ||||
| β-gal− vs NeuN+: p = 0.515 | ||||
| Adora2a | Adenosine A2a receptor; found in D2-expressing striatal neurons | 25369 | −3.0-fold | F(2,8) = 2.690, p = 0.128 |
| β-gal+ vs β-gal−: p = 0.204 | ||||
| β-gal+ vs NeuN+: p = 0.051 | ||||
| β-gal− vs NeuN+: p = 0.342 | ||||
| Kcnc1 | Kv4; Kv3.1; Potassium voltage-gated channel, Shaw-related subfamily, member 1 | 25327 | −2.6-fold | F(2,9) = 16.942, p < 0.005 |
| β-gal+ vs β-gal−: −2.2-fold; n = 4, 5; p < 0.01 | ||||
| β-gal+ vs NeuN+: −3.1-fold; n = 4, 3; p < 0.001 | ||||
| β-gal− vs NeuN+: −1.4-fold; p = 0.031 | ||||
| Map2k6 | Mkk6; Mitogen-activated protein kinase kinase 6; activator of p38 MAPK | 114495 | −5.2-fold | F(2,9) = 17.196, p < 0.005 |
| β-gal+ vs β-gal−: −7.9-fold; n = 4, 5; p < 0.05 | ||||
| β-gal+ vs NeuN+: −15.1-fold; n = 4, 3; p < 0.001 | ||||
| β-gal− vs NeuN+: −1.9-fold; p = 0.013 |
One-way ANOVA (F and p values are shown in Quantitative PCR column) was used to compare gene expression in three groups of cells: β-gal-positive neurons and β-gal-negative neurons from the same rats after a single injection of cocaine, and all neurons (NeuN-positive; NeuN+) from different rats after a single injection of saline. Fold-level changes are relative to control levels in NeuN + cells. Positive fold-level changes indicate higher levels, while negative fold-level changes indicate lower levels, than in NeuN + control samples.