Number of bouts of the five vigilance states during the 12 h day and night periods
| Group | Number of bouts | |||||
|---|---|---|---|---|---|---|
| Cataplexy | REM sleep | Wake | Non-REM sleep | Sleep attacks | ||
| During the night | ||||||
| 1 | WT (n = 5) | NA | 17.60 (1.60) | 81.6 (12.37)‡ | 80.40 (11.85) | NA |
| 2 | No rAAV (n = 7) | 16.36 (2.28) | 30.50 (7.57) | 115.64 (9.28) | 88.14 (10.31) | 19.71 (3.44) |
| 3 | rAAV-GFP (n = 10) | 17.15 (2.89) | 23.35 (3.37) | 130.9 (10.71) | 92.95 (12.67) | 23.1 (3.34) |
| 4 | rAAV-orexin in striatum (n = 9) | 16.44 (3.58) | 25.11 (4.75) | 129.56 (8.09) | 81.72 (10.98) | 36.33 (7.48)§ |
| 5 | rAAV-MCH-orexin (30% of MCH neurons) (n = 9) | 21.89 (4.06) | 27.56 (4.32) | 120.56 (7.30) | 78.72 (10.26) | 26.28 (4.36) |
| 6 | rAAV-MCH-orexin (65% of MCH neurons) (n = 7) | 28.21 (4.22) | 27.07 (3.05) | 148.64 (11.37) | 81.50 (5.81) | 47.71 (8.19)§§ |
| 7 | Dilute rAAV-orexin in LH (n = 6) | 8.67 (1.35) | 21.92 (2.87) | 122.67 (8.84) | 98.08 (9.01) | 17.50 (2.39) |
| 8 | rAAV-orexin in LH (n = 11) | 3.96 (0.56)* | 37.73 (1.87)† | 114.05 (7.03) | 96.68 (5.70) | 17.18 (3.22) |
| 9 | rAAV-orexin in ZI (n = 8) | 6.06 (1.33)** | 35.88 (4.06) | 123.13 (6.14) | 107.50 (7.08) | 11.94 (1.65) |
| During the day | ||||||
| 1 | WT (n = 5) | NA | 62.40 (3.45) | 149.80 (8.47) | 151.50 (8.13) | NA |
| 2 | No rAAV (n = 7) | 6.64 (2.29) | 77.43 (4.61) | 169.50 (7.06) | 168.50 (5.16) | 15.79 (3.08) |
| 3 | rAAV-GFP (n = 10) | 3.34 (0.75) | 53.25 (6.34)‖ | 153.7 (10.32) | 152.10 (11.68) | 4.15 (1.24) |
| 4 | rAAV-orexin in striatum (n = 9) | 4.56 (0.86) | 62.78 (4.03) | 166.94 (4.74) | 159.17 (3.74) | 11.83 (2.04) |
| 5 | rAAV-MCH-orexin (30% of MCH neurons) (n = 9) | 3.17 (0.49) | 80.44 (5.74) | 153.78 (7.85) | 157.11 (9.21) | 6.89 (2.01) |
| 6 | rAAV-MCH-orexin (65% of MCH neurons) (n = 7) | 4.93 (1.44) | 63.00 (7.01) | 144.00 (6.71) | 136.86 (8.57) | 9.36 (2.41) |
| 7 | Dilute rAAV-orexin in LH (n = 6) | 0.833 (0.28)¶ | 65.92 (5.38) | 169.50 (6.96) | 171.25 (6.75) | 2.67 (0.76) |
| 8 | rAAV-orexin in LH (n = 11) | 1.409 (0.43)¶ | 76.50 (3.96) | 156.09 (7.22) | 156.00 (6.99 | 10.55 (1.97) |
| 9 | rAAV-orexin in ZI (n = 8) | 0.625 (0.23)¶ | 79.19 (5.01) | 165.38 (6.99) | 167.50 (6.98) | 3.63 (0.72) |
Values represent number ± SEM. Each vigilance state was identified, and the average number during the 12 h day and night periods was determined for each group. Cataplexy and sleep attacks did not occur in WT mice and is not applicable (NA).
↵*Versus 2–6,
↵**versus 2, 3, 5, 6;
↵†versus 1;
↵‡versus 3, 4, 6, 9;
↵§versus 8, 9;
↵§§versus 2, 3, 5, 7–9 (p < 0.02);
↵¶versus 2 (p < 0.001);
↵‖versus 2, 5, 8, 9 (p < 0.02).