Percentage of sleep–wake states during 12 h day and night cycles
| Group | Cataplexy | REM | Wake | Non-REM sleep | Sleep attacks | |
|---|---|---|---|---|---|---|
| Night | ||||||
| 1 | WT (n = 5) | NA | 2.40 (0.18) | 75.01 (1.62) | 22.51 (1.65) | NA |
| 2 | No-rAAV (n = 7) | 3.16 (0.62) | 4.64 (0.67) | 67.06 (2.27) | 23.37 (1.91) | 1.76 (0.35) |
| 3 | rAAV-GFP (n = 10) | 2.96 (0.57) | 3.32 (0.46) | 67.08 (2.87) | 24.84 (2.89) | 1.80 (0.31) |
| 4 | rAAV-orexin in striatum (n = 9) | 3.76 (0.81) | 3.25 (0.67) | 66.45 (1.96) | 23.60 (2.26) | 2.93 (0.46)*** |
| 5 | rAAV-MCH-orexin (30% MCH neurons) (n = 9) | 3.88 (0.61) | 3.46 (0.60) | 69.30 (2.64) | 21.07 (2.51) | 2.28 (0.46) |
| 6 | rAAV-MCH-orexin (65% MCH neurons) (n = 7) | 4.06 (0.71) | 2.68 (0.41) | 70.02 (2.12) | 19.90 (2.29) | 3.35 (0.49)** |
| 7 | Dilute rAAV-orexin (n = 6) | 1.66 (0.22) | 2.89 (0.35) | 70.57 (2.67) | 23.64 (2.64) | 1.25 (0.21) |
| 8 | rAAV-orexin in LH (n = 11) | 0.49 (0.06)* | 4.49 (0.26) | 66.17 (1.49) | 27.55 (1.17) | 1.30 (0.25) |
| 9 | rAAV-orexin in ZI (n = 8) | 0.77 (0.18)* | 4.43 (0.37) | 64.62 (2.42) | 29.17 (2.19) | 1.01 (0.15) |
| Day | ||||||
| 1 | WT (n = 5) | NA | 9.11 (0.52) | 36.13 (0.56) | 54.74 (0.98) | NA |
| 2 | no-rAAV (n = 7) | 0.92 (0.34) | 10.03 (0.39) | 37.31 (0.54) | 50.56 (0.73) | 1.18 (0.28) |
| 3 | rAAV-GFP (n = 10) | 0.79 (0.19) | 6.32 (0.57)§‡ | 43.15 (2.87) | 49.44 (2.62) | 0.30 (0.09) |
| 4 | rAAV-orexin in striatum (n = 9) | 0.92 (0.15) | 6.78 (0.45)§ | 37.13 (1.47) | 54.24 (1.37) | 0.93 (0.19) |
| 5 | rAAV-MCH-orexin (30% MCH neurons) (n = 9) | 0.48 (0.08) | 7.7 (0.71) | 39.38 (1.37) | 51.90 (1.38) | 0.55 (0.21) |
| 6 | rAAV-MCH-orexin (65% MCH neurons) (n = 7) | 0.63 (0.19) | 5.55 (0.77)§‡ | 43.53 (2.23) | 49.55 (2.12) | 0.74 (0.20) |
| 7 | Dilute rAAV-orexin (n = 6) | 0.15 (0.05)§ | 7.45 (0.59) | 41.66 (1.29) | 50.56 (1.26) | 0.17 (0.04) |
| 8 | rAAV-orexin in LH (n = 11) | 0.16 (0.06)§ | 8.44 (0.50) | 40.50 (1.99) | 50.13 (1.77) | 0.77 (0.15) |
| 9 | rAAV-orexin in ZI (n = 8) | 0.08 (0.04)§† | 7.99 (0.58) | 40.13 (2.26) | 51.56 (1.97) | 0.24 (0.06) |