F values obtained from the two-way ANOVAs for FosB/ΔFosB expression in each brain area for experiments 1–4
| Brain area | Experiment 1: effect of previous housing on FosB expression during SD | Experiment 2: effect of IL lesion and enriched housing on FosB expression during SD | Experiment 3: effect of IL lesion and non-enriched housing on FosB expression during SD | Experiment 4: effect of ectopic lesions on FosB expression during SD | ||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|---|
| Housing (H) ANOVA F(2,36), p | Defeat stress (S) ANOVA F(1,36), p | Interaction (H × S) ANOVA F(2,36), p | IL infusion (L) ANOVA F(1,30), p | Housing (H) ANOVA F(2,30), p | Interaction (L × H) ANOVA F(2,30), p | IL infusion (L) ANOVA F(1,30), p | Housing (H) ANOVA F(2,30), p | Interaction (L × H) ANOVA F(2,30), p | Infusion (L) ANOVA F(2,30), p | Time of infusion (T) ANOVA F(1,30), p | Interaction (L × T) ANOVA F(2,30), p | |
| PL | 27.2, p < 0.0001 | 15.16, p < 0.001 | 9.62, p < 0.004 | 4.24, p < 0.05 | 7.64, p < 0.002 | 15.18, p < 0.001 | ||||||
| IL | 65.65, p < 0.0001 | 32.75, p < 0.0001 | 5.23, p < 0.05 | 145.2, p < 0.0001 | 48.32, p < 0.0001 | 5.28, p < 0.01 | 6.24, p < 0.005 | |||||
| Acb | 100.1, p < 0.0001 | 103.17, p < 0.0001 | 28.14, p < 0.002 | 18.37, p < 0.0001 | 7.34, p < 0.005 | 40.95, p < 0.0001 | ||||||
| CG | 34.81, p < 0.0001 | 10.13, p < 0.01 | 4.97, p < 0.05 | 4.96, p < 0.05 | 41.52, p < 0.0001 | 4.33, p < 0.02 | 17.23, p < 0.0001 | 30.92, p < 0.001 | ||||
| PIR | 39.91, p < 0.0001 | 23.91, p < 0.0001 | 9.95, p < 0.01 | |||||||||
| PVN | 13.31, p < 0.0001 | 48.76, p < 0.0001 | 9.114, p < 0.001 | 8.00, p < 0.009 | 10.89, p < 0.0031 | 12.69, p < 0.0001 | 18.37, p < 0.0001 | |||||
| ITC | 19.947, p < 0.0005 | 7.826, p < 0.01 | 17.67, p < 0.002 | 9.719, p < 0.0006 | 5.17, p < 0.001 | 15.03, p < 0.001 | 4.182, p < 0.03 | |||||
In experiment 1, the two-way ANOVA examined the interaction between home-cage housing (enriched vs standard vs impoverished) and social defeat exposure (SD exposure vs home-cage housing) on FosB/ΔFosB expression in the listed areas. The two-way ANOVA for experiment 2 examined the interaction between IL infusion (ibotenic acid vs vehicle) and housing treatment (ILinf→EE, ILinf→EE→SD, and EE→ILinf→SD) on FosB/ΔFosB expression in the listed areas. The two-way ANOVA for experiment 3 examined the interaction between IL infusion (ibotenic acid vs vehicle) and housing treatment (ILinf→SE, ILinf→SE→SD, and SE→ILinf→SD) on FosB/ΔFosB expression in the listed areas. In experiment 4, the two-way ANOVA examined the interaction between CG/PL infusions (ibotenic acid vs vehicle) and infusion timing (before vs after EE) on FosB/ΔFosB expression in the listed areas. Only statistically significant effects and interactions are included. Nonsignificant analyses have been left blank.