Pair | Mutants | TBOA | Asp |
---|---|---|---|
1 | 355-279 | 16.0 ± 0.07 (15.6–16.4) | 8.9 ± 0.01 (8.3–9.7) |
2 | 355-129 | 8.6 ± 0.03 (8.0–9.2) | 18.6 ± 0.04 (17.9–19.3) |
3 | 353-130 | 12.5 ± 0.05 (11.5–13.5) | 14.9 ± 1.0 (13.0–16.8) |
Due to the mobility of the spin probes, the deconvolution results in a distribution of distances between the two spin labels. Therefore, spin–spin distances (in Å) are given as the center of the distance distributions with errors and the width of the distance distributions (in parentheses). The data was obtained by deconvolution of the single- and double-labeled spectra in Figure 5 (see Materials and Methods). Asp, l-aspartate.