Table 1.

List of primary antibodies

AntigenAntigen detailsHostClonalityDilutionSupplierCatalog no.Purification methodReference/specificity
CCKGastrin-17MouseMonoclonal1:1000Dr. G. Ohning (CURE, University of California, Los Angeles, Los Angeles, CA)9303Ascites fluidsRecognizes gastrin and CCK, but gastrin is absent of telencephalon (Ohning et al., 1996)
CRRecombinant rat calretininRabbitPolyclonal1:1000MilliporeAB5054AntiserumWestern blot shows a single band at 32 kDa molecular weight (manufacturer). Labeling pattern as published in previous reports (Fonseca et al., 1995)
CoupTFIIRecombinant human COUP-TF II (amino acids 43–64)MouseMonoclonal1:200Perseus Proteomics; clone H7147PP-H7147-00Ascites fluidsTested by Western blot and KO animal (Qin et al., 2007)
GFPRecombinant GFPChickenPolyclonal1:2000Aves LabsGFP-1020IgY fractionNo immunohistochemistry signal can be detected from the GFP-negative Cre+;Z/EG−/− mouse tissue
PVPurified frog muscle parvalbuminMouseMonoclonal1:1000Sigma-Aldrich; clone PARV-19P3088Ascites fluidsLabeling pattern as with rabbit antiserum (Swant; PV-28) (Kubota et al., 2011)
PVRat muscle parvalbuminRabbitPolyclonal1:1000SwantPV-28AntiserumNo labeling in KO mouse (Schwaller et al., 1999)
M2RAmino acids 225–359RatMonoclonal1:500Millipore; clone M2-2-B3MAB367Protein A purifiedLevey et al., 1995
nNOSRecombinant human neuronal nitric oxide synthaseRabbitPolyclonal1:1000MilliporeAB5380Peptide affinity chromatographyLabeling pattern as published with other antibodies (Fuentealba et al., 2008)
ReelinRecombinant reelin amino acids 164–496MouseMonoclonal1:1000Millipore; clone G10MAB5364Protein A purifiedRecognizes the H epitope near the N terminus of the protein (de Bergeyck et al., 1998). No staining in the reeler mouse (de Bergeyck et al., 1997)
SOMSynthetic cyclic (1–14) somatostatin conjugated to bovine thyroglobulinRatMonoclonal1:200Millipore; clone YC7MAB354Culture supernatantLabeling pattern as published with other antibodies (Eyre et al., 2009)
SOMSame as the rat antibodyRabbitPolyclonal1:500DakoA0566AntiserumLabeling pattern as published with the rat antibody (our observation)
VIPPorcine VIPGuinea pigPolyclonal1:1000Peninsula LaboratoriesT-5030AntiserumLabeling pattern as published with other antibodies (Eyre et al., 2009)