Table 1.

Dose–response data for RDL with unnatural amino acids at positions 206 and 254

MutantLog EC50 (m)EC50m)nHn
WT−4.909 ± 0.34171.4 ± 0.27
F206A>3004
F206Y−6.941 ± 0.090.112.2 ± 0.36
F206-FPhe−4.190 ± 0.05651.8 ± 0.35
F206-F2Phe−3.257 ± 0.055541.8 ± 0.36
F206-F3Phe−2.990 ± 0.0410222.0 ± 0.46
Y254ANF8
Y254F−4.050 ± 0.05762.1 ± 0.44
Y254-FPhe−3.995 ± 0.071101.3 ± 0.46
Y254F2-Phe−2.135 ± 0.087331.1 ± 0.24
Y254F3-Phe−1.599 ± 0.0725181.2 ± 0.37
F206Y/Y254F−5.754 ± 0.071.81.9 ± 0.57
F146A−4.140 ± 0.12732.1 ± 0.25
F146F3Phe−4.084 ± 0.15821.4 ± 0.44
  • All values are expressed as mean and SEM.