Experimental designs for Experiments 1, 2, and 3
| Experiment/group | Day 1 | Day 2 | Day 3 | Day 4 | Day 5 |
|---|---|---|---|---|---|
| Experiment 1 | Conditioning | Extinction | Test | ||
| Vehicle | 5 CS+ | 6 CS− → Vehicle | CS− | — | — |
| FGF2 | 5 CS+ | 6 CS− → FGF2 | CS− | — | — |
| FGF2 No Extinction | 5 CS+ | — → FGF2 | CS− | — | — |
| Experiment 2 | Conditioning | Extinction day 1 | Extinction day 2 | Test | |
| Vehicle | 5 CS+ | 12 CS− → Vehicle | 12 CS− | CS− Context A/B | — |
| FGF2 | 5 CS+ | 6 CS− → FGF2 | — | CS− Context A/B | — |
| Experiment 3 | Conditioning | Extinction day 1 | Extinction day 2 | Reinstatement | Test |
| Vehicle Reinstate | 5 CS+ | 12 CS− → Vehicle | 12 CS− | Unsignaled US | CS− |
| FGF2 Reinstate | 5 CS+ | 6 CS− → FGF2 | — | Unsignaled US | CS− |
| Vehicle No Reinstate | 5 CS+ | 12 CS− → Vehicle | 12 CS− | — | CS− |