Table 3.

Statistical details

PFCLIMMTSSVIS
Statistical results for injections at E70
    Number of plotted cells
        BrdU
            Total113692899512062307
            Mean227.2185.6199.0241.2461.4
            SEM13.016.112.012.815.0
            PFC0.03180.15740.33920.0015Multiple comparisons (p values–LSD contrast)
            LIM0.41120.0037<0.0001
            MT0.0237<0.0002
            SS0.0140
        [3H]dT
            Total566687800761551
            Mean113.2137.4160.0152.2110.2
            SEM10.416.526.89.029.9
    BrdU–[3H]dT difference in number of plotted cells
        p0.00010.07190.22150.0005<0.0001ANOVA, single factor (t test)
    BrdU–[3H]dT difference in cell numbers, corrected for section cut thickness and [3H]dT detection abilitya
        p0.00020.00030.0021<0.00010.5679
    Comparison of the percentage distributions of BrdU versus [3H]dT
        χ27.7410.526.158.163.61Kruskal–Wallis test
        p0.00540.00120.01320.00430.0575
        F (p)0.35 (0.0066)0.26 (0.0006)0.38 (0.0111)0.43 (0.0265)0.27 (0.0007)F test of dispersion
    Correlation coefficient of percentage of BrdU versus [3H]dT
        rs, p0.83, p < 0.00010.77, p < 0.00010.82, p < 0.00010.79, p < 0.00010.69, p < 0.0001Spearman's rank
    Comparison of the percentage distributions of BrdU versus [3H]dT, corrected for cortical thicknessb
        χ24.086.111.43.581.22Kruskal–Wallis test
        p0.04340.01350.23650.05830.2691
        F (p)2.04 (0.0594)2.28 (0.0298)1.50 (0.2839)1.33 (0.4528)3.02 (0.0040)F test of dispersion
    Correlation coefficient for mean percentage distributions of BrdU versus [3H]dTb
        rs, p0.89, p < 0.00010.68, p < 0.00010.78, p < 0.00010.65, p < 0.00010.88, p < 0.0001Spearman's rank
Statistical results for injections at E55
    Number of plotted cells
        BrdU
            Total779110512199941593
            Mean194.8276.3304.8248.5398.3
            SEM34.245.923.334.946.3
        [3H]dT
            Total484328428189442
            Mean161.3109.3142.763.0147.3
            SEM14.29.922.312.211.8
    Comparison of the percentage distributions of BrdU versus [3H]dT
        χ22.372.750.355.178.19Kruskal–Wallis test
        p0.12380.09740.55410.02300.0042
        F (p)0.42 (0.0234)0.28 (0.0009)0.20 (<0.0001)0.34 (0.0047)0.13 (0.0001)F test of dispersion
    Correlation coefficient of the percentage of BrdU versus [3H]dT
        rs, p0.67, p < 0.00010.90, p < 0.00010.86, p < 0.00010.60, p = 0.00050.61, p = 0.0004Spearman's rank
    Correlation coefficient of the percentage of BrdU versus [3H]dTb
        rs, p0.89, p < 0.00010.59, p = 0.00050.79, p < 0.00010.81, p < 0.00010.88, p < 0.0001Spearman's rank
  • Only essential details are shown for each group, and not all details are shown for E55, which follows the same trends observed for E70.

  • aCorrected by increasing [3H]dT cell numbers by 500%. This corresponds to the case for which we had the worse possible comparisons (Table 2, Total-3, for the number of [3H]dT cells; i.e., the case in which we assume [3H]dT cells are detected within 10 μm). For the other two cases, the estimated numbers of [3H]dT cells are so high compared with the number of BrdU cells that all per corresponding area comparisons were always statistically significant.

  • bAfter normalizing cortical thickness per area against that in BrdU material.

  • Values in bold are statistically significant.