Labeled cell numbers and percentages | |||||
---|---|---|---|---|---|

PFC | LIM | MOT | SOM | VIS | |

Injections at E70 | |||||

Number of labeled cells | |||||

BrdU | |||||

Total | 1136 | 928 | 995 | 1206 | 2307 |

% Dead | 59.9 | 73.0 | 75.1 | 68.3 | 16.3 |

[^{3}H]dT^{a} | |||||

Total-1 | 11,320 | 13,740 | 16,000 | 15,220 | 11,020 |

Total-2 | 5660 | 6870 | 8000 | 7610 | 5510 |

Total-3 | 2830 | 3435 | 4000 | 3805 | 2755 |

% Dead | 0.0 | 0.0 | 0.0 | 0.0 | 0.0 |

Percentage largest accumulation of labeled cells within a single 100 μm bin | |||||

BrdU | |||||

% | 12.4 | 17.4 | 12.7 | 16.2 | 13.4 |

Depth (μm) | 250 | 250 | 250 | 250 | 1050 |

[^{3}H]dT | |||||

% | 21.0 | 20.2 | 14.5 | 16.2 | 36.8 |

Depth (μm) | 650 | 350 | 450 | 550 | 850 |

Percentage largest accumulation of labeled cells within a single 100 μm bin, corrected for cortical thickness^{b} | |||||

BrdU | |||||

% | 12.2 | 13.2 | 12.5 | 15.7 | 15.7 |

Depth (μm) | 250 | 250 | 250 | 250 | 1150 |

[^{3}H]dT | |||||

% | 16.6 | 12.7 | 10.0 | 12.8 | 28.9 |

Depth (μm) | 950 | 450 | 650 | 350 | 1250 |

Percentage accumulation of labeled cells within 5 and 10 (100 μm) bins | |||||

BrdU | |||||

5 bins | 48.2 | 44.1 | 45.8 | 49.6 | 89.3 |

10 bins | 79.3 | 61.7 | 70.3 | 72.8 | 99.6 |

[^{3}H]dT | |||||

5 bins | 77.4 | 81.4 | 64.8 | 70.4 | 54.6 |

10 bins | 99.8 | 96.9 | 96.1 | 95.5 | 85.0 |

Percentage accumulation of labeled cells within 5 and 10 (100 μm) bins, corrected for cortical thickness^{b} | |||||

BrdU | |||||

5 bins | 47.3 | 49.0 | 45.9 | 51.2 | 57.1 |

10 bins | 79.4 | 64.7 | 69.6 | 73.1 | 85.6 |

[^{3}H]dT | |||||

5 bins | 67.1 | 56.8 | 43.4 | 55.8 | 86.2 |

10 bins | 97.9 | 87.2 | 73.1 | 90.4 | 95.3 |

Percentage accumulation of labeled cells deeper than 1900 μm from pia (deep bins) | |||||

BrdU | |||||

% | 4.8 | 21.8 | 14.7 | 15.8 | 0.2 |

%^{b} | 4.7 | 22.0 | 14.1 | 15.6 | 0.2 |

[^{3}H]dT | |||||

% | 0.0 | 0.0 | 0.4 | 1.1 | 0.0 |

%^{b} | 0.0 | 0.0 | 8.1 | 0.9 | 0.0 |

Injections at E55 | |||||

Number of labeled cells | |||||

BrdU | |||||

Total | 779 | 1105 | 1219 | 994 | 1593 |

Mean | 194.8 | 276.3 | 304.8 | 248.5 | 398.3 |

% Dead | 75.9 | 49.5 | 57.3 | 21.1 | 45.9 |

[^{3}H]dT^{a} | |||||

Total | 2420 | 1640 | 2140 | 945 | 2210 |

Mean | 806.7 | 546.7 | 713.3 | 315.0 | 736.7 |

Percentage largest accumulation of labeled cells within a single 100 μm bin, corrected for cortical thickness^{b} | |||||

BrdU | |||||

% | 13.2 | 9.1 | 5.3 | 11.3 | 12.8 |

Depth (μm) | 1050 | 1050 | 1850 | 1150 | 1350 |

[^{3}H]dT | |||||

% | 20.7 | 22.2 | 9.8 | 22.3 | 39.4 |

Depth (μm) | 750 | 950 | 1450 | 750 | 1150 |

Percentage accumulation of labeled cells deeper than 1900 μm from pia (deep bins) | |||||

BrdU | |||||

%^{b} | 4.2 | 2.9 | 35.5 | 10.1 | 8.5 |

[^{3}H]dT | |||||

%^{b} | 0.0 | 0.0 | 24.1 | 0.0 | 0.0 |

Percentage accumulation within 5 and 10 bins is not shown for injections at E55; they follow the same trends of those shown for injections at E70.

↵

Corrected by increasing [^{a}^{3}H]dT cell numbers as follows: total-1 detected cell numbers multiplied 20× represents the case in which detection occurred on the top 2.5 μm of the tissue; total-2 (10×) and total-3 (5×) represent cases in which detection occurred within 5 and 10 μm from the top, correspondingly. For [^{3}H]dT, we assumed survival of 100% until detection; for details, see Results, and Materials and Methods, Technical considerations and corrections of methodological differences.↵

After normalizing cortical thickness per area against that in BrdU material.^{b}