Table 2.

Details on labeled cell numbers and percentage comparisons

Labeled cell numbers and percentages
PFCLIMMOTSOMVIS
Injections at E70
    Number of labeled cells
        BrdU
            Total113692899512062307
            % Dead59.973.075.168.316.3
        [3H]dTa
            Total-111,32013,74016,00015,22011,020
            Total-256606870800076105510
            Total-328303435400038052755
            % Dead0.00.00.00.00.0
    Percentage largest accumulation of labeled cells within a single 100 μm bin
        BrdU
            %12.417.412.716.213.4
            Depth (μm)2502502502501050
        [3H]dT
            %21.020.214.516.236.8
            Depth (μm)650350450550850
    Percentage largest accumulation of labeled cells within a single 100 μm bin, corrected for cortical thicknessb
        BrdU
            %12.213.212.515.715.7
            Depth (μm)2502502502501150
        [3H]dT
            %16.612.710.012.828.9
            Depth (μm)9504506503501250
    Percentage accumulation of labeled cells within 5 and 10 (100 μm) bins
        BrdU
            5 bins48.244.145.849.689.3
            10 bins79.361.770.372.899.6
        [3H]dT
            5 bins77.481.464.870.454.6
            10 bins99.896.996.195.585.0
    Percentage accumulation of labeled cells within 5 and 10 (100 μm) bins, corrected for cortical thicknessb
        BrdU
            5 bins47.349.045.951.257.1
            10 bins79.464.769.673.185.6
        [3H]dT
            5 bins67.156.843.455.886.2
            10 bins97.987.273.190.495.3
    Percentage accumulation of labeled cells deeper than 1900 μm from pia (deep bins)
        BrdU
            %4.821.814.715.80.2
            %b4.722.014.115.60.2
        [3H]dT
            %0.00.00.41.10.0
            %b0.00.08.10.90.0
Injections at E55
    Number of labeled cells
        BrdU
            Total779110512199941593
            Mean194.8276.3304.8248.5398.3
            % Dead75.949.557.321.145.9
        [3H]dTa
            Total2420164021409452210
            Mean806.7546.7713.3315.0736.7
    Percentage largest accumulation of labeled cells within a single 100 μm bin, corrected for cortical thicknessb
        BrdU
            %13.29.15.311.312.8
            Depth (μm)10501050185011501350
        [3H]dT
            %20.722.29.822.339.4
            Depth (μm)75095014507501150
    Percentage accumulation of labeled cells deeper than 1900 μm from pia (deep bins)
        BrdU
            %b4.22.935.510.18.5
        [3H]dT
            %b0.00.024.10.00.0
  • Percentage accumulation within 5 and 10 bins is not shown for injections at E55; they follow the same trends of those shown for injections at E70.

  • aCorrected by increasing [3H]dT cell numbers as follows: total-1 detected cell numbers multiplied 20× represents the case in which detection occurred on the top 2.5 μm of the tissue; total-2 (10×) and total-3 (5×) represent cases in which detection occurred within 5 and 10 μm from the top, correspondingly. For [3H]dT, we assumed survival of 100% until detection; for details, see Results, and Materials and Methods, Technical considerations and corrections of methodological differences.

  • bAfter normalizing cortical thickness per area against that in BrdU material.