Antibodies used for the labeling of cortical synaptic boutons
| Anatomical marker | Primary antibody | Serum used for blocking step and antibody dilutions | Secondary antibody |
|---|---|---|---|
| VAChT (expressed in cholinergic varicosities) | Goat anti-VAChT; 1:10,000 (Millipore), polyclonal | Normal rabbit serum (Jackson ImmunoResearch) | Biotinylated rabbit anti-goat; 1:200 (Vector Labs) |
| TH (expressed in both dopaminergic and noradrenergic varicosities) | Rabbit anti-TH; 1:3000 (Millipore), polyclonal | Normal goat serum (Jackson ImmunoResearch) | Alexa Fluor 488-conjugated goat anti-rabbit; 1:800 (Invitrogen) |
| DBH (expressed in noradrenergic varicosities) | Mouse anti-DBH; 1:25 (MediMabs), monoclonal | Normal donkey serum (Jackson ImmunoResearch) | Rhodamine Red X-conjugated donkey anti-mouse; 1:200 (Jackson ImmunoResearch) |
| VGluT1 (expressed in glutamatergic varicosities) | Mouse anti-VGluT1; 1:100 (characterized in Bell et al. (2006); MediMabs), monoclonal | Normal donkey serum (Jackson ImmunoResearch) | Alexa Fluro 488-conjugated donkey anti-mouse; 1:800 (Invitrogen) |
| GAD 65 (expressed in GABAergic varicosities) | Mouse anti-GAD-65; 1:1000 (Millipore), monoclonal | Normal donkey serum (Jackson ImmunoResearch) | Alexa Fluor 488-conjugated donkey anti-mouse; 1:800 (Invitrogen) |
| Neurotrophic TrkA high-affinity mNGF receptor (expressed on cholinergic axon) | Polyclonal goat anti-TrkA; 1:100 (R&D Systems) | Normal donkey serum (Jackson ImmunoResearch) | Rhodamine Red X-conjugated donkey anti-goat; 1:200 (Jackson ImmunoResearch) |