Analysis of P2X2/5 receptors containing trafficking mutant subunits HEK cells transfected with P2X2, P2X2-K366A, P2X5, and P2X5-K372A subunits or the different combination
| IATP, pA/pF | I/IP2X2, % | IBzATP, pA/pF | IBzATP/IATP | n | |
|---|---|---|---|---|---|
| P2X2 | −845 ± 76 | 100 | −416 ± 76 | 0.48 ± 0.04 | 18 |
| P2X5 | −2.1 ± 0.9 | 0.27 | — | — | 16 |
| P2X2-K366A | −1.5 ± 0.5 | 0.15 | — | — | 6 |
| P2X2-K366A + P2X5 | −10.2 ± 1.6 | 1.1 | −2.8 ± 0.4 | 0.35 ± 0.04* | 23 |
| P2X2 + P2X5 | −390 ± 75 | 45.5 | −112 ± 29 | 0.26 ± 0.02** | 14 |
| P2X2 + P2X5-K372A | −446 ± 123 | 51.6 | −104 ± 25 | 0.30 ± 0.03* | 9 |
Currents were evoked by BzATP (300 μm) and ATP (100 μm). No response to BzATP was detected for P2X5 or P2X2-K366A. IBzATP/IATP was calculated using the averaged responses to each agonist from individual cells. Results are shown as mean ± SEM.
↵*p < 0.05,
↵** p < 0.01 compared to P2X2, ANOVA followed by Tukey–Kramer multiple comparison test.