Primary and secondary antibodies used in the analysis
| Antigen | Species | Dilution, 1: | Source |
|---|---|---|---|
| Notch1 (intracellular domain) | Rabbit | 1000 | Nyfeler et al., 2005 |
| Notch1 (extracelluar domain) | Rat | 100 | H. R. MacDonald |
| Notch2 (extracelluar domain) | Rat | 100 | H. R. MacDonald |
| Notch3 | Goat | 100 | R&D Systems |
| bGal | Rabbit | 2000 | CAPPEL |
| PCNA | Mouse | 1000 | Dako North America |
| Doublecortin | Guinea pig | 2000 | Chemicon |
| Sox2 | Rabbit | 500 | Chemicon |
| BrdU | Rat | 2000 | Serotec |
| Ascl1 (Mash1) | Mouse | 200 | F. Guillemot |
| GFAP | Mouse | 500 | Sigma-Aldrich |
| Neuronal nuclear antigen (NeuN) | Mouse | 500 | Sigma |
| FITC/Cy3/Cy5 conjugated anti-mouse, rabbit, rat, goat | Donkey | 1000 | Jackson Immunoresearch |