Table 1.

General health, reflexes, sensory abilities, motor functions

Genotypes+/+ (N = 19)+/− (N = 27)−/− (N = 22)
Fur condition (3 point scale)222
Bald patches (%)5.200
Missing whiskers (%)000
Piloerection (%)3.73.722.7
Body tone (3 point scale)222
Limb tone (3 point scale)222
Physical abnormalities (%)000
Body weight (g) males26.8 ± 0.8528.7 ± 0.6527.2 ± 0.92
Body weight (g) females21.2 ± 0.3621.7 ± 0.3220.7 ± 0.45
Body temperature (°C)32.3 ± 0.3132.5 ± 0.2933.0 ± 0.32
Empty cage behavior
    Transfer freezing (%)5.3013.6
    Wild running (%)000
    Stereotypies (%)000
    Exploration (3 point scale)222
Motoric abilities
    Trunk curl (%)94.796.3100
    Wire hang (latency) (s)54.2 ± 5.7858.2 ± 1.8252.8 ± 4.80
Reflexes
    Forepaw reach (%)100100100
    Righting reflex (%)10010095.5
    Corneal (%)100100100
    Pinna (%)100100100
    Vibrissae (%)100100100
Reactivity
    Auditory startle (%)94.710095.5
    Struggle (%)31.633.331.8
    Dowel biting (%)0.58 ± 0.160.63 ± 0.120.68 ± 0.17
Grip strengthN = 9N = 12N = 14
108.4 ± 4.8109.3 ± 2.1107.1 ± 3.6
Open-field explorationN = 11N = 15N = 17
    Total distance1646.6 ± 153.01512.7 ± 99.01371.6 ± 78.1
    Horizontal activity3133.5 ± 207.92994.5 ± 128.82561.1 ± 127.3*
    Vertical activity104.2 ± 13.3101.6 ± 10.7102.5 ± 10.7
    Center time (s)89.6 ± 7.6100.2 ± 7.7106.8 ± 9.6
Foot print testN = 11N = 11N = 9
    Forepaw width1.43 ± 0.111.58 ± 0.081.44 ± 0.12
    Hindpaw width2.35 ± 0.142.39 ± 0.122.39 ± 0.08
    Stride length6.97 ± 0.176.65 ± 0.197.55 ± 0.19
  • Normal general health, neurological reflexes, grip strength, locomotor activities, and gait functions were seen in adults of all genotypes of Shank3 mice. Data in Table 1 display the absence of genotype differences in general health measures for Cohort 1, with the exception of grip strength. Similarly, no genotype differences were detected across the three genotypes on measures of general health and neurological reflexes in Cohort 2, or in either genotype of the WT-HET cohort. Cohort 2 and the WT-HET cohort were not tested for footprint gait. Genotype differences were found on several measures of open field locomotor activity in Cohort 2 (see Results for F and p values).

  • *p < 0.05 compared with +/+.