Quantification of dye coupling in Cx30−/−/Cx47−/−, Cx30−/−/Cx47+/−, and Cx30+/−/Cx47−/− mice compared to Cx30+/−/Cx47+/− and wild-type controls at P10–P15 in the corpus callosum
| Corpus callosum | Wild type | Cx30+/−/Cx47+/− | Cx30−/−/Cx47+/− | Cx30+/−/Cx47−/− | Cx30−/−/Cx47−/− |
|---|---|---|---|---|---|
| Networks of coupled cells (%) | 62 (n = 21) | 53 (n = 15) | 75 (n = 16) | 47 (n = 15) | 74 (n = 19) |
| Extent of tracer spread (μm) | 99 ± 61 (n = 13) | 111 ± 62 (n = 8) | 113 ± 46 (n = 12) | 156 ± 58 (n = 7) | 125 ± 71 (n = 14) |
| Number of coupled cells per network | 15 ± 13 | 14 ± 10 | 13 ± 8 | 14 ± 8 | 11 ± 9 |
| CNPase+ cells per network (%) | 83 (71–92) | 91 (67–98) | 68 (45–87) | 100 (100–100)* | 100 (100–100)*** |
| eGFP+ cells per network (%) | – | 91 (67–98) | 68 (45–87) | 100 (100–100)# | 100 (100–100)### |
| CNPase−/GFAP− cells per network (%) | 5 (0–13) | 0 (0–0) | 0 (0–0)† | 0 (0–0) | 0 (0–0)†† |
| GFAP+ cells per network (%) | 6 (0–12) | 9 (2–33) | 32 (13–55) | 0 (0–0)‡ | 0 (0–0)‡‡,‡‡‡ |
Networks are shown as percentages of biocytin-injected oligodendrocytes found coupled to at least one adjacent cell. Data regarding the extent of tracer spread and the number of coupled cells are reported as the average ± SD. Quantification of the population of coupled cells (CNPase+, eGFP+, CNPase−/GFAP−, GFAP+) is indicated as median (25th–75th percentiles). n, Number of experiments.
↵*p < 0.05, Cx30+/−/Cx47−/− versus Cx30+/+/Cx47+/+ and Cx30−/−/Cx47+/− (Mann–Whitney U test; Kruskal–Wallis test, p < 0.001); ****p < 0.001, Cx30−/−/Cx47−/− versus wild type, Cx30+/−/Cx47+/−, and Cx30−/−/Cx47+/− (Mann–Whitney U test, Kruskal–Wallis test, p < 0.001).
↵#p < 0.05, Cx30+/−/Cx47−/− versus Cx30+/−/Cx47+/− and Cx30−/−/Cx47+/− (Mann–Whitney U test; Kruskal–Wallis test, p < 0.001); ###p < 0.001, Cx30−/−/Cx47−/− versus Cx30+/−/Cx47+/− and Cx30−/−/Cx47+/− (Mann–Whitney U test; Kruskal–Wallis test, p < 0.001).
↵†p < 0.05, Cx30−/−/Cx47+/− versus Cx30+/+/Cx47+/+ (Mann–Whitney U test; Kruskal–Wallis test, p < 0.001); ††p < 0.05, Cx30−/−/Cx47−/− versus Cx30+/+/Cx47+/+ (Mann–Whitney U test; Kruskal–Wallis test, p < 0.001).
↵‡p < 0.05, Cx30+/−/Cx47−/− versus Cx30−/−/Cx47+/− (Mann–Whitney U test; Kruskal–Wallis test, p < 0.001); ‡‡p < 0.05, Cx30−/−/Cx47−/− versus Cx30+/+/Cx47+/+ (Mann–Whitney U test; Kruskal–Wallis test, p < 0.001); ‡‡‡p < 0.001, Cx30−/−/Cx47−/− versus Cx30+/−/Cx47+/− and Cx30−/−/Cx47+/− (Mann–Whitney U test; Kruskal–Wallis test, p < 0.001).