Primers and probe for semi-quantitative RT-PCR
| Gene | Sequence | Position | Product length |
|---|---|---|---|
| Cx43 (gja1) | Sense 5′-TTTGACTTCAGCCTCCAAGGA | −80 | 79 bp |
| Antisense 5′-TCTGGGCACCTCTCTTTCACTTA | −24 | ||
| Cx43 (probe) | Sense FAM-5′-TTCCACCACTTTGGCGTGCCG-TAMRA | −58 |
Position 1 is the first nucleotide of the initiation codon. The fluorogenic TaqMan Cx43 probe was labeled at their 5′ end with 6-carboxyfluorescein (FAM) and at their 3′ end with 6-carboxytetramethylrhodamine (TAMRA). The GenBank accession number for Cx43 (gja1) is NM_010288.