Table 2.

The expected distributions for the number of steps calculated using the binomial distribution for different scenarios of channel stoichiometry

Steps
12345
β-VFP
    Data36%62%2%0%0%
    Expected for 2 β-subunits and p = 78%36%64%0%0%0%
    Expected for 3 β-subunits and p = 78%11%41%48%0%0%
α-VFP
    Data18%30%43%6%3%
    Expected for 3 α-subunits, p = 78%, homomers = 15%9%36%44%6%5%
    Expected for 2 α-subunits, p = 78%, homomers = 15%31%55%3%6%5%
  • The probability for VFP to be fluorescent was set as 78%. In the case of α1-VFP:β channels, the percentage of homomers was additionally set as 15%. Comparison of data with different scenarios of heteromeric GlyR stoichiometry.