Table 1.

In vitro/in vivo characteristics of GCaMP5 variants

GCaMP variantMutationsaMax. in vitro ΔF/FFbase, Fmax HEKbRatio (Fmax/Fbase) HEKbBaseline brightness cultured neurons (%)cCa2+ affinity (Kd)pKa (sat/apo)Hill coefficientkond
GCaMP25.1 ± 0.1NDNDND545 ± 32 nm7.02 ± 0.01/8.72 ± 0.021.8 ± 0.1ND
GCaMP3Deletion R2, M65K, T115V, N362D12.3 ± 0.41 ± 0.032.9 ± 0.1100 ± 1.6405 ± 9 nm6.97 ± 0.01/8.40 ± 0.022.1 ± 0.18.3 ± 1.5 s−1
2.9 ± 0.1133 ± 3 s−1
GCaMP5AD380Y17.4 ± 1.20.95 ± 0.073.8 ± 0 0.0257.3 ± 1.0307 ± 12 nm6.77 ± 0.02/8.70 ± 0.042.7 ± 0.1ND
3.7 ± 0.15
GCaMP5BL59H, E60P23.8 ± 3.40.48 ± 0.023.9 ± 0.235.2 ± 0.8NDNDNDND
1.87 ± 0.09
GCaMP5CL59Q, E60P35.1 ± 0.90.49 ± 0.024.5 ± 0.1NDNDNDNDND
2.2 ± 0.08
GCaMP5DT302L, R303P22.0 ± 1.80.34 ± 0.023.3 ± 0.137.5 ± 0.5730 ± 18 nm7.43 ± 0.02/8.91 ± 0.052.5 ± 0.17.8 ± 1.5 s−1
1.1 ± 0.375.3 ± 2.6 s−1
GCaMP5EL59H, E60P, T302L, R303P40.9 ± 2.20.19 ± 0.0110.8 ± 0.330.1 ± 0.4NDNDNDND
2.0 ± 0.14
GCaMP5FL59Q, E60P, T302L, R303P162 ± 3.50.17 ± 0.037.1 ± 0.3NDNDNDNDND
1.2 ± 0.13
GCaMP5GT302L, R303P, D380Y32.7 ± 1.50.40 ± 0.055.5 ± 0.461.6 ± 1.3460 ± 11 nm6.96 ± 0.02/9.14 ± 0.052.5 ± 0.26.5 ± 1.0 s−1
2.1 ± 0.1158.1 ± 1.1 s−1
GCaMP5HL59Q, E60P, T302L, R303P, D380Y158 ± 120.01 ± 0.0148.6 ± 35.3NDNDNDNDND
0.44 ± 0.02
GCaMP5IL59H, E60P, T302L, R303P, D380Y42.0 ± 3.10.03 ± 0.0228.9 ± 19.239.7 ± 6.5NDNDNDND
0.71 ± 0.02
GCaMP5JL59H, E60P, D380YNDNDND42.2 ± 1.4NDNDND
GCaMP5KD380Y, R392G9.4 ± 0.14NDND197.2 ± 7.3189 ± 5 nmND3.8 ± 0.3ND
GCaMP5LA52V, T302L, R303P17.7 ± 0.3NDNDND390 ± 18 nmNDNDND
GCaMP2-LIAE61IA31.2 ± 0.8NDNDNDNDNDNDND
GCaMP3-KFL59K, E60F9.2 ± 0.4NDNDNDNDNDNDND
  • aGCaMP5s also contain the mutations R2 deletion, M65K, T115V, and N362D listed for GCaMP3.

  • bFbase, Fmax, and Ratio are from the acetylcholine assay.

  • cValues ± SEM, baseline brightness of GCaMP3 set to 100%.

  • dFirst values are measured at a calcium concentration of 670 nm, second at a calcium concentration of 8.52 μm. ND, not determined.