Physiological properties of the recorded interneurons
| Fast-spiking basket cells (n = 10) | Axo-axonic cells (n = 10) | Bistratified cells (n = 10) | O-LM cells (n = 10) | |
|---|---|---|---|---|
| Input resistance (MΩ) | 70.82 (60.94–88.64)a | 111.41 (70.96–137.1) | 117.26 (91.01–146.6) | 136.8 (86.59–199) |
| Membrane time constant (ms) | 9.17 (7.06–11.83) | 10.58 (9.39–11.41) | 9.68 (8.71–14.32) | 18.14 (12.58–25.58) |
| Membrane capacitance (pF) | 119 (84–141) | 89 (81–125) | 97 (79–103) | 144 (124–159) |
| Relative sag amplitude | 0.191 (0.139–0.217) | 0.168 (0.133–0.176) | 0.092 (0.078–0.167)b | 0.500 (0.396–0.761) |
| AP amplitude (mV) | 61.3 (53.9–63.3) | 65.3 (60.6–70.5) | 64.4 (59.8–77) | 72.8 (57.8–78) |
| AP width at half-maximum (ms) | 0.4 (0.4–0.5) | 0.5 (0.4–0.5) | 0.4 (0.3–0.5) | 0.6 (0.5–0.8) |
| AHP amplitude (mV) | 15.5 (12.8–18) | 14.8 (8.6–16.7)c | 17.9 (16–20.5) | 18.9 (16.6–22) |
| Spike frequency (Hz) at small current stepsd | 51 (50–64) | 43 (41–83) | 65 (58–101) | 29 (18–49) |
| Accommodation index at small current stepsd | 1.67 (1.33–1.93) | 2.02 (1.49–3) | 1.61 (1.5–1.9) | 1.66 (1.52–2.28) |
| Spike frequency (Hz) at large current stepse | 61 (50–84) | 77 (56–114) | 66 (41–101) | 65 (51–78) |
| Accommodation index at large current stepse | 1.76 (1.54–1.93) | 1.8 (1.16–3.28) | 1.94 (1.5–2.41) | 1.9 (1.55–2.82) |
Data are presented as the median and the interquartile range in parentheses. Values indicated with bold differ significantly (p < 0.05) from all other cell types, while values in italics indicate significant differences from two other cell types. AP, Action potential; AHP, afterhyperpolarization.
↵aFS BC versus AAC, p = 0.143; FS BC versus BIST, p = 0.015; FS BC versus O-LM, p = 0.002.
↵bBIST versus AAC, p = 0.222; BIST versus BC, p = 0.022; BIST versus O-LM, p < 0.001.
↵cAAC versus FS BC, p = 0.356; AAC versus BIST, p = 0.043; AAC versus O-LM, p = 0.015.
↵dVoltage responses at which interneurons were capable to discharge continuously during the entire trace upon the smallest current injection (150–400 pA range for FS IN and 50–250 pA for O-LM cells) were compared.
↵eVoltage responses at which interneurons fired with the highest frequency were compared.