Table 1.

Kinetic parameters of Kv7.2/3 currents

Expressed constructsV1/2 (mV)aSlope (zF/RT)aActivation constant (ms)bDeactivation constant (ms)c
X. laevis oocytes
    Kv7.2/3−31.6 ± 0.6 (n = 57)8.8 ± 0.3 (n = 57)102.8 ± 12.2 (n = 11)55.3 ± 3.5 (n = 10)
    Kv7.2/3 + SGK1.1−31.1 ± 0.6 (n = 60)9 ± 0.3 (n = 60)140.4 ± 25.7 (n = 8)42.6 ± 5.9 (n = 4)
HEK293
    Kv7.2/3−21.27 ± 1.8 (n = 11)13.8 ± 1.3 (n = 11)80.4 ± 13.5 (n = 11)43.3 ± 10.1 (n = 10)
    Kv7.2/3 + SGK1.1−19.43 ± 1.2 (n = 14)14.98 ± 0.5 (n = 14)72.3 ± 8.7 (n = 14)49.2 ± 6.4 (n = 12)
  • a Half-maximal activation voltage calculated from Boltzmann equation fits to activation curves (Fig. 1B,E).

  • b Calculated from monoexponential fits to evoked currents at + 40 mV from −80 mV (oocytes) or −30 mV (HEK293).

  • c Calculated from monoexponential fits to evoked currents at −100 mV from holding potentials of + 50 mV (oocytes) or −30 mV (HEK293).