Breathing effects elicited by photostimulating ChR2-expressing RVLM-CA neurons in conscious mice
| N | Baseline VT (μl/g) | Stimulated VT (μl/g) | Baseline fR (bpm) | Stimulated fR (bpm) | Baseline MV (μl/g/min) | Stimulated MV (μl/g/min) | |
|---|---|---|---|---|---|---|---|
| ChR2 vector | 9 | 8.5 ± 0.5 | 8.0 ± 0.4*** | 121.6 ± 5.4 | 164.5 ± 6.4 | 1014 ± 41 | 1309 ± 87* |
| Control vector | 6 | 8.8 ± 0.9 | 8.7 ± 0.9 | 111.4 ± 8.4 | 114.5 ± 9.3 | 971 ± 105 | 978 ± 111 |
Results analyzed by two-way ANOVA. Breathing frequency (fR): p = 0.009 for the interaction between ChR2 and stimulation; tidal volume (VT): p = 0.74 for the interaction between ChR2 and stimulation; minute volume (MV): p = 0.11 for the interaction between ChR2 and stimulation, p = 0.038 for the effect of CHR2.
↵*p < 0.05,
↵***p < 0.001, Bonferroni post-test comparison between baseline and stimulation.