Disruption of the SorCS1c YAQM motif decreases APP localization to the TGN
| pCDNA | SORCS1 | SORCS1Y1132A | SORCS1M1135A | |
|---|---|---|---|---|
| TGN38 | 33 ± 5% | 52 ± 4% | 40 ± 3% | 40 ± 3% |
| EEA1 | 39 ± 4% | 36 ± 5% | 37 ± 2% | 42 ± 7% |
| RAB7 | 68 ± 5% | 40 ± 4% | 48 ± 5% | 29 ± 3% |
Colocalization was quantified using ImageJ and the JACoP plugin (Bolte et al., 2006). The percentage of colocalization is expressed as the percentage of APP pixel overlap with each of the organelle markers ± SEM.