Table 2.

Summary of synaptic response from WT, Munc13-2 KO, Munc13-3 KO, Munc13-2-3 DKO, and overexpression of Munc13-1(1–451) calyx of Held synapse

GenotypeValues ± SEM (n)Statistical testp value
P9–P11 synapse
    eEPSC amplitude (nA)
        WT1.47 ± 0.13 (28)Student's t testp > 0.05
        Munc13-2 KO1.25 ± 0.08 (23)
        WT1.57 ± 0.16 (17)Student's t testp > 0.05
        Munc13-3 KO2.02 ± 0.20 (17)
        WT (a)1.88 ± 0.15 (19)Kruskal–Wallis test*p > 0.05 (a vs b)
        Munc13-2-3 DKO (b)2.00 ± 0.20 (18)p < 0.0001 (b vs c)
        Munc13-1(1–451) (c)0.59 ± 0.06 (18)
    Cumulative EPSC amplitude (nA)
        WT4.00 ± 0.34 (16)Student's t testp > 0.05
        Munc13-2 KO4.07 ± 0.38 (11)
        WT4.40 ± 0.42 (12)Student's t testp > 0.05
        Munc13-3 KO5.14 ± 0.53 (8)
        WT (a)6.32 ± 0.44 (15)Kruskal–Wallis test*p > 0.05 (a vs b)
        Munc13-2-3 DKO (b)6.15 ± 0.50 (17)p < 0.0001 (b vs c)
        Munc13-1(1–451) (c)2.72 ± 0.24 (16)
P18–P21 synapse
    eEPSC amplitude (nA)
        WT4.94 ± 0.40 (21)One-way ANOVAp > 0.05
        Munc13-2 KO5.78 ± 0.52 (23)
        Munc13-3 KO5.96 ± 0.71 (14)
        WT (a)5.29 ± 0.51 (24)Kruskal–Wallis test*p > 0.05 (a vs b)
        Munc13-2-3 DKO (b)5.80 ± 0.49 (24)p < 0.01 (b vs c)
        Munc13-1(1–451) (c)2.57 ± 0.32 (15)
    Cumulative EPSC amplitude (nA)
        WT20.08 ± 2.76 (16)One-way ANOVAp > 0.05
        Munc13-2 KO20.64 ± 2.07 (18)
        Munc13-3 KO21.88 ± 2.65 (12)
        WT (a)26.48 ± 2.01 (22)Kruskal–Wallis test*p > 0.05 (a vs b)
        Munc13-2-3 DKO (b)25.97 ± 3.58 (20)p < 0.001 (b vs c)
        Munc13-1(1–451) (c)10.97 ± 1.26 (14)
  • *Kruskal–Wallis test with a post hoc Dunn's test to compare selected pairs.