Groups and procedures
| Animals | n (offspring/treatment) | Measures |
|---|---|---|
| Group 1 | 7–8 (P15), 4 (P40) | Peptide mRNA expression: PVN, PFLH, CeA |
| Group 2 | 4–8 (P15) | Density of peptide-expressing neurons: PVN, PF, LH, CeA |
| Group 3 | 4 (P15) | New cell generation (BrdU+) |
| Group 4 | 4 (P15) | Phenotype of newly generated cells: neurons, astrocytes (BrdU+, NeuN+, GFAP+, double-labeling) |
| Group 5 | 4 (P15) | Phenotype of newly generated cells: microglia (BrdU+, Iba-1+, double-labeling) |
| Group 6 | 4 (P15) | Apoptotic cell death (TUNEL+) |
| Group 7 | 4 (P15) | Genesis of peptide-expressing neurons (BrdU+/peptide+ double-labeling) |
| Group 8 | 6–8 (P40) | Behavior: consumption of nicotine, ethanol, fat |
Each individual offspring was examined only once, with one offspring taken from each litter and the number of offspring/group equal to the number of litters.