Membrane properties of mature D1 and D2 MSNs in primary culturesa
| Nontreated | Treated | |||
|---|---|---|---|---|
| D1 MSN | D2 MSN | D1 MSN | D2 MSN | |
| Resting membrane potential (mV) | −73.9 ± 1.8 (n = 22) | −75.5 ± 1.5 (n = 43) | −75.1 ± 5.2 (n = 9) | −73.9 ± 1.4 (n = 38) |
| Input resistance (mΩ) | 265 ± 18 (n = 19) | 215 ± 23 (n = 42) | 268 ± 27 (n = 18) | 209 ± 20 (n = 42) |
| Capacitance (pF) | 54 ± 5 (n = 16) | 43 ± 4 (n = 23) | 52 ± 4 (n = 13) | 67 ± 5 (n = 19)***,+ |
| Inward rectification index | 1.7 ± 0.1 (n = 8) | 1.7 ± 0.1 (n = 22) | 2.0 ± 0.2 (n = 8) | 2.1 ± 0.1 (n = 13) |
↵aWhole-cell recordings from identified MSNs in nontreated and treated conditions showed that chronic D2R activation increased the capacitance and inward rectification index in only D2 MSNs. D1 MSNs remained unchanged despite the treatment, serving as an internal control (data derived from at least 4 culture preparations).
↵***p < 0.001, comparing same cell type between treatment conditions using an unpaired t test;
↵+p < 0.05 comparing different cell types within a treatment condition using an unpaired t test.